Making STEM Specimen We will look at each one of these methods in turn.

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Presentation transcript:

Making STEM Specimen We will look at each one of these methods in turn.

Making STEM Specimen – Dispersion Method

Making STEM Specimen – Microtomy Method

Making S(T)EM Specimen – Focus Ion Beam (FIB), Ion Mill, Plasma/UV Cleaner Method

Making STEM Specimen – Focus Ion Beam (FIB) Method

Making a Thin Cross-sectional Specimen by FIB

The FIB can weld any two pieces of material together at the nanoscale Welding specimen to 33mm Cu grid

Limited Micro/Nano Machining MEMS Lab on a chip

Viewing of Cell Cluster

Cell Tissue (No Fixation) Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK Tobacco flower petals

Cell Tissue (No Fixation) Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK Tobacco flower petals Metal deposition

Cell Tissue (No Fixation) Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK Tobacco flower petals Features never before seen were found in these specimens.

Making STEM Specimen – Focus Ion Beam (FIB) Method