Introduction to Instruments FUJI DRI-CHEM 4000i Scil Vet Focus 5 Cryostat for Frozen Sections 2010/12/06 Speaker：Le-Shi Tsai

Notice for the use of animal center Instrument Please attend illustration meeting before you begin operating instruments. If you do not participate in the illustration meeting , you may NOT use the instrument. (The meeting will be held 2-3 times per semester, and please pay attention to the animal center announcement.) Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation. illustration meeting 1 or 2 days prior to use (as the instrument may be) before must be filled Registration forms for the instrument is ready to facilitate the managers Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation

Charging methods Instrument Charging methods $150/0.5hr FUJI DRI-CHEM 4000i Biochemistry Analyzer *Less than 3 tests : First hour - $ 400/hour Additional hours-$ 200 /hour *More than 3 tests : Each one more test, the first hour charge extra $100 Scil Vet Focus 5 Hematology Analyzer 1 test - $750 each additional test -$100 Leica CM3050 S Cryostat for Frozen Sections $150/0.5hr Less than 3 tests the first hour and $ 400, the beginning of the second hour, $ 200 per hour. More than three hours overcharged 100 yuan more than a first. Each one more test first hour overcharged 100 Charge $100 in the first hour

Biochemistry Analyzer FUJI DRI-CHEM 4000i Biochemistry Analyzer

Main instrument Need to purchase reagents by user The registration forms Exception code table Need to purchase reagents by user Main instrument

Colorimetric Potentiometric Linear Measurement time

External Structure of the Instrument Light source 廢料盒 Can not open the lid when indication light is on

Internal Structure of the Instrument

Automatic detection 1. can be automatically diluted 2. A test only needs at least 10μL. （ Manual detection ）

Principle：Colorimetric Spreading layer： filtered out macromolecules (proteins, dyes, etc.) evenly spread the fluid sample into the reaction layer β-D-glucose＋O2＋H2O GOD D-gluconic acid＋H2O2 1,7-Dihydroxynaphthalene＋4-aminoantipyrine＋H2O2 POD Red dye 505nm reflection density calibration curve glucose concentration

Principle：Potentiometric generated voltage difference between ion selective electrodes applied to a reference solution and to the sample potentiometric difference between the sample and the reference solution is measured E = E0 + 2.303 * RT/Nf * Log ai ai = f * Ci Ci = Ion concentration

Scil Vet Focus 5 Hematology Analyzer

Introduction to Instrument The following animals can be detected: dog、 rat、 pig、rabbit、mouse、 G. pig The following items can be detected: WBC、DC、RBC、HGB、HCT、MCV、MCH、MCHC、RDW、Retics、PLT、MPV、PCT Slide 002

Equipment the registration forms the kit printer waste pail main Instrument

Equipment 1.Diluent 2.Clean Deproteinizer 3.Cellyse the kit 1. red blood cell lysis 2. reagent to convert all hemoglobin to the stable cyanmethemoglobin form

Principles 感 應 區 Cells are injected into the core of a A distinguishing feature of blood cell types is the diameter of the cells.（RBC、WBC、Platelet） 2. WBCs are classified according to the characteristics of their cytoplasm and nucleus. 3. Cyanmethemoglobin can then be detected by a spectrophotometer set at 540 nm. The concentration of hemoglobin is then calculated from the optical density of the solution. 感 應 區 The cells are forced to pass through aperture in single file (one by one). Cells are injected into the core of a sheath flow and confined to a narrow single-file stream by hydrodynamic focusing(15μm). Diluent form a vortex

Specimen preparation 1. Use the EDTA anticoagulant 3 ml tubes. Specimen requirements 1. Use the EDTA anticoagulant 3 ml tubes. 2. Specimen volume: At least 1.5 ml 3. Specimen volume are less than 1.5mL(at least 100 μL): Use small test tube containing a specimen and add EDTA as an anti-coagulant (Manual mode). Preservation conditions Samples can be analyzed within 4 hour(room temperature). Interfering factors Measurement results are affected by hemolysis, chyle and clotted blood. DO NOT use clotted blood specimen.

Automatic Mode open the lid mix the specimen well put your sample into the specimen- container housing rack

Cryostat for Frozen Sections Leica CM3050 S Cryostat for Frozen Sections

Frozen Tissue Processing tissue process and cutting fixing and embedding only use O.C.T as embedding medium freeze and slice Hard tissue using a dedicated film stain mounting observe it under a microscope

The advantage of Leica CM3050 S It is ideal for undecalcified hard tissue : 1. can be set to any section thickness and section automatically 2. with a dedicated film, can avoid the hard tissue broken. with rough-cut function adjustable specimen temperature： In accordance with the type of specimen to select the appropriate temperature. adjustable cutting speed

Specifications Maximum 40mm 0.5~300μm 0.1~210mm/s -10℃~-50 ℃ Specimen size Maximum 40mm Section thickness setting 0.5~300μm Sectioning speed 0.1~210mm/s Specimen temperature -10℃~-50 ℃

supplies you need to purchase Consumables Suitable specimens Stage Buy different size according to the size of the specimen Disposable blades General tissue Ukrainian carbon blades Hard tissue Dedicated film

Thanks for your attention