BIO 225 Microbiology Ken Malachowsky

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Presentation transcript:

BIO 225 Microbiology Ken Malachowsky Florence-Darlington Technical College

MICROSCOPE SPECIMEN Magnifies Illuminates Contrasts Resolves Optics Distinguishes Lens systems Background Ability to Vary Light intensity Condenser OCULAR OBJECTIVE Provide sharp, crisp image Lamp Distinguish Total magnification Two close objects Iris diaphragm Multiply two powers

Leewenhoek’s Simple Microscope

A Compound Brightfield Microscope

Scanning Power X 4x

Low Power X X 4x 10x

High Power X X 4x 10x 40x 4x 10x 40x 100x Field of Vision

Depth Perception Steve Berg winona state university, used with permission

Refraction

The use of Immersion Oil

Microscope Rules Never force anything on the microscope Inform the instructor if there is a problem with the microscope Only use your assigned microscope Please do the following when you are finished Click the 4x objective in place Lower the stage far from the objective Place the microscope in its assigned space

Slides to View Letter E Colored Threads Cheek cells Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wetmount KM

Cheek Cells 100x 400x KM

Slides to View Letter E Colored Threads Cheek cells Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wetmount KM

Cyclops 100x KM

Daphnia 100x KM

Volvox 100x 400x KM

Spirogyra 400x KM

Amoeba 100x 400x KM

Paramecium 100x 400x KM

Representative algae, or plantlike protists: The diatom Campylodiscus hibernicus These protists usually have cell walls surrounded by a loosely attached test that contains silicon or calcium carbonate. Most reproduce by binary fission. Some lack flagella.

Diatoms 400x KM

Mixed Bacteria 1000x

Slides to View Letter E Colored Threads Cheek cells Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wet mount