Overview of ATP Production Presented by: Professor Steven P. Dion – Salem State College Sport, Fitness & Leisure Studies Dept.

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Presentation transcript:

Overview of ATP Production Presented by: Professor Steven P. Dion – Salem State College Sport, Fitness & Leisure Studies Dept.

ATP Production - Dion2 ATP Production Before cells can use the energy of sunlight or energy /calories stored in carbohydrates, they must transfer the energy to molecules of ATP. Before cells can use the energy of sunlight or energy /calories stored in carbohydrates, they must transfer the energy to molecules of ATP. –ATP is composed of adenine, ribose, and three phosphate groups. –ATP transfers energy to many different chemical reactions; almost all metabolic pathways directly or indirectly run on energy supplied by ATP.

ATP Production - Dion3 ATP Production –ATP can donate a phosphate group (phosphorylation) to another molecule, which then becomes primed and energized for specific reactions. (ready to be used for energy)

ATP Production - Dion4 ATP Production In human cells, cellular respiration releases energy from energy-rich organic molecules and changes ADP into ATP. In human cells, cellular respiration releases energy from energy-rich organic molecules and changes ADP into ATP. –Aerobic respiration is the main ATP-producing pathway –Anaerobic respiration produces much less ATP (because no oxygen is involved) and can only be used for short periods of time, such as in vigorous muscle exercise.

ATP Production - Dion5 Aerobic Respiration Initial Breakdown of Glucose Initial Breakdown of Glucose –Glycolysis reactions occur in the cytoplasm (liquid stuff outside the nucleus) and results in the breakdown of glucose to pyruvate; small amounts of ATP are generated. –Glucose is first phosphorylated in energy- requiring steps then split to form two molecules of PGAL.

ATP Production - Dion6 Aerobic Respiration –By substrate-level phosphorylation, four ATP are produced; but because two ATP were used previously, there is a net gain of only two ATP. –Enzymes remove H+ and electrons from PGAL to change NAD to NADH (which is used later in oxidative phosphorylation). The end products of glycolysis are: two pyruvates, two ATP (net gain), and two NADH for each glucose molecule degraded. The end products of glycolysis are: two pyruvates, two ATP (net gain), and two NADH for each glucose molecule degraded.

ATP Production - Dion7 The Krebs Cycle and Preparatory Steps Krebs cycle (occurring in the mitochondria) degrades pyruvate to carbon dioxide, water, ATP, H+ ions, and electrons. Krebs cycle (occurring in the mitochondria) degrades pyruvate to carbon dioxide, water, ATP, H+ ions, and electrons. Pyruvate (produced in the cytoplasm) enters the mitochondria and is converted to acetyl- CoA, which then joins oxaloacetate already present from a previous "turn" of the cycle. Pyruvate (produced in the cytoplasm) enters the mitochondria and is converted to acetyl- CoA, which then joins oxaloacetate already present from a previous "turn" of the cycle.

ATP Production - Dion8 Krebs cycle serves three functions: Krebs cycle serves three functions: –H+ and e– are transferred to NAD+ and FAD. –Two molecules of ATP are produced by substrate-level phosphorylation. –Most of the molecules are recycled to conserve oxaloacetate for continuous processing of acetyl-CoA.

ATP Production - Dion9 The final stage of aerobic respiration occurs in the electron systems embedded in the inner membrane of the mitochondrion. The final stage of aerobic respiration occurs in the electron systems embedded in the inner membrane of the mitochondrion. –Oxidation phosphorylation (which takes place on the cristae of the mitochondria) processes the H+ ions and electrons to generate high yields of ATP. –NADH and FADH2 give up their electrons to transport (enzyme) systems embedded in the mitochondrial inner membrane.

ATP Production - Dion10 –The actual ATP synthesis is accomplished when H ions that have been pumped out of the inner mitochondrial compartment flow back through a channel protein called ATP synthase.

ATP Production - Dion11 Oxygen joins with the "spent" electrons and H+ to yield water. Oxygen joins with the "spent" electrons and H+ to yield water. –The production of ATP is completely dependent on the supply of oxygen that withdraws the electrons at the end of the transport systems.

ATP Production - Dion12 Glucose Breakdown Net ATP Yield of Aerobic Respiration Net ATP Yield of Aerobic Respiration –The aerobic route is summarized: –C6H12O6 + 6O2 ——> 6CO2 + 6H2O –Electron transport yields thirty-two ATP; glycolysis yields two ATP; Krebs yields two ATP for a grand total of thirty-six ATP per glucose molecule. The actual yield can vary with cell type. The actual yield can vary with cell type.

ATP Production - Dion13 ATP From Anaerobic Pathways Anaerobic pathways operate when oxygen is absent (or limited); pyruvate from glycolysis is metabolized to produce molecules other than acetyl-CoA. Anaerobic pathways operate when oxygen is absent (or limited); pyruvate from glycolysis is metabolized to produce molecules other than acetyl-CoA. In lactate fermentation, glycolysis produces two pyruvate, two NADH molecules, two ATP molecules, and two lactate, which tend to build up and cause temporary muscle cramps. In lactate fermentation, glycolysis produces two pyruvate, two NADH molecules, two ATP molecules, and two lactate, which tend to build up and cause temporary muscle cramps.

ATP Production - Dion14 The ADP/ATP Cycle The ADP/ATP cycle is a method for renewing the supply of ATP that is constantly being used up in the cell. The ADP/ATP cycle is a method for renewing the supply of ATP that is constantly being used up in the cell. Energy input couples inorganic phosphate to ADP to form energized ATP. Energy input couples inorganic phosphate to ADP to form energized ATP.

ATP Production - Dion15 Alternative Energy Sources Carbohydrates Carbohydrates Lipids Lipids Proteins Proteins

ATP Production - Dion16 Alternative Energy Sources Carbohydrates are the body’s first source of energy. Carbohydrates are the body’s first source of energy. –Excess carbohydrate intake is stored as glycogen in liver and muscle for future use. –Free glucose is used until it runs low; then glycogen reserves are tapped.

ATP Production - Dion17 Energy From Fats Lipids are used when carbohydrate supplies run low. Lipids are used when carbohydrate supplies run low. –Excess fats are stored away in cells of adipose tissue. –Fats are digested into glycerol (which enters glycolysis) and fatty acids, which enter the Krebs cycle. –Because fatty acids have many more carbon and hydrogen atoms, they are degraded more slowly and yield greater amounts of ATP.

ATP Production - Dion18 Energy From Proteins Proteins are used as the last resort for supplying energy for the body. Proteins are used as the last resort for supplying energy for the body. –Amino acids are released by digestion and travel in the blood. –After the amino group is removed, the amino acid remnant is fed into the Krebs cycle.