Lecture 6 Basic Statistics Dr. A.K.M. Shafiqul Islam School of Bioprocess Engineering University Malaysia Perlis 28.09.2011.

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Presentation transcript:

Lecture 6 Basic Statistics Dr. A.K.M. Shafiqul Islam School of Bioprocess Engineering University Malaysia Perlis

CALIBRATION CURVE The straight-line equation given by where y = is the dependent variable x = is the independent variable m = is the slope of the curve b = is the intercept on the ordinate (y axis); y is usually the measured variable, plotted as a function of changing x.

CALIBRATION CURVE

The correlation coefficient is used as a measure of the correlation between two variables The closer the observed values to the most probable values, the more definite is the relationship between x and y. It gives numerical measures of the degree of correlation.

CALIBRATION CURVE The Pearson correlation coefficient is one of the most convenient to calculate. This is given by where r is the correlation coefficient, n is the number of observations, s x is the standard deviation of x, s y is the standard deviation of y, x i and y j are the individual values of the variables, Y and y are their means.

CALIBRATION CURVE The use of differences in the calculation is frequently cumbersome, This equation can be transformed to a more convenient form:

CALIBRATION CURVE Correlation coefficient is calculated for a calibration curve to ascertain the degree of correlation between the measured instrumental variable and the sample concentration. General rule, 0.90 < r < 0.95 indicates a fair curve, 0.95 < r < 0.99 a good curve, and r > 0.99 indicates excellent linearity. An r > can sometimes be obtained with care.

Data for Example 3.19 Sample Your method(mg/dL) Standard method(mg/dL) XyXy A B C D E F CALIBRATION CURVE

Solution CALIBRATION CURVE

A more conservative measure of closeness of fit is the square of the correlation coefficient, r 2, and most statistical programs calculate this value An r value of 0.90 corresponds to an r 2 value of only 0.81, This is also called the coefficient of determination. CALIBRATION CURVE

Thank you