Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development Jana Klásková Laboratory of Growth Regulators.

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Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development Jana Klásková Laboratory of Growth Regulators

Cytokinin metabolism

Cytokinin degradation

IIINhibitors of CYtokinin DEgradation Zatloukal et al., 2008 LGR-2679 LGR-2678

Study of inhibitors in biological assays CKX assay

Study of inhibitors in biological assays II Receptor assay (CRE1/AHK4) Receptor assay (AHK3)

Study of inhibitors in biological assays III ARR5:GUS assay

Effect of INCYDE-(F) application by spraying CONTROL 10uM INCYDE-F CONTROL 10uM INCYDE CONTROL 10uM INCYDE-F 10uM INCYDE

Effect of INCYDE-F application by spraying Low temperature (15°C) for 4 weeks CONTROL 1 uM 5 uM 10 uM

Effect of LGR-2679 application by drain CONTROL 0,1uM X 1x 0,1uM X 3x 0,1uM X 5x 1uM X 1x 1uM X 3x 1uM X 5x CONTROL 0,1uM X 1x 0,1uM X 3x 0,1uM X 5x 1uM X 1x 1uM X 3x 1uM X 5x CONTROL LGR 2679; 0,1uM LGR 2679; 1uM CONTROL 1x3x5x 1x3x5x

Effect of LGR-2679 application by drain 0,1uM X 3x 0,1uM X 5x 0,1uM X 8x 0,1uM X 3x 0,1uM X 5x 0,1uM X 8x CONTROL 0,1uM X 3x 0,1uM X 5x 0,1uM X 8x 1uM X 3x 1uM X 5x 1uM X 8x CONTROL LGR 2679; 0,1uM LGR 2679; 1uM 3x5x8x 3x5x8x

Mgr. Lukáš Spíchal, Ph.D. Mgr. Ota Blahoušek Mgr. Markéta Gemrotová, Ph.D Mgr. Jaroslav Nisler, Ph.D Thank you for your attention

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