Chanleakhena Phoeung, Aung Naing Myo, Stéphane Bayen, Boon Huan Tan, Nicolas Steenkeste, Khov Meas, Puy Lim, Monidarin Chou, Barry Kelly Symposium on Biodiversity & Health Symposium th November 2014
Outline Introduction Exposure to Pesticides Objective and Method of study Results of study Conclusion Recommendations
Source : Nile River Awareness Kit "Physical and Chemical Characteristics of Water Quality."Physical and Chemical Characteristics of Water Quality Introduction
Problems bioaccumulation and Biomagnification
Detection the presence of frequently used pesticides in fish collected from Tonle Sap lake in Cambodia Target compounds : 65 POPs – Organochlorines (OCPs) – Polychlorinate biphenyls (PCBs) – Bis-(p-chlorophenyl) sulfone (BCPS) – …… Objective of Study
Sampling sites : Siem Reap, Pursat, K. Thom, K. Chhnang 76 fishes collected 36 samples analyzed -12 (12 species)from Siem Reap -15 (12 species)from Pursat -4 ( 4 species) from K. Thom -5 (5 species) from K. Chhnang Study sites and sample collection TS1 TS2 TS3 TS4 Chong Kneas Phat Sanday KamPong Luong Chnok Trou
Gel permeation chromatography (GPC) with organic solvents followed by Florisil Clean-up Samples processing
Injector : splitless mode Gaz : He Column : DB- 5 MS 60 m X 0.25 mm X 0,10 µm T ˚ column : 80˚ C Inj. Vol : 3 ml/min, T ˚ inj : 250 ˚C Flow rate : 1,2 ml/min Presure : 155,7 kPa 1min 80˚C 160˚C 20 ˚C/min 200 ˚C 4 ˚C/min 250 ˚C 2 ˚C/min 20 ˚C/min 10min Gradient 300˚C 8 GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) : Condition Samples Analysis
RESULTS
Samples characteristic To tal TS TS TS TS To tal
Main POPs detected Compound nameConcentrationPermissible limit p,p’- DDT0.00 to 3.70 ng/g5ppm PCBs0.00 to 9.99 ng/g2ppm BCPS0.00 to 0.86 ng/g10ppm FAO J Toxicol Environ Health A Jun 11;57(3): J Toxicol Environ Health A.
Result of DDT Samples Concentration (ng/g)
Result of PCBs Samples Mean of Concentration (ng/g)
Result of BCPS Concentration (ng/g) Samples
POPs detected in different sites Study sites
Conclusion Trace of dangerous compounds are detected in Tonle Sap fish Predominant compounds are p,p’-DDT, PCBs and BCPS. Concentration of each compound is variable from one site to another These compounds are bioaccumulated and biomagnified it may affect to the people. Limit use of dangerous pesticides by educating users about its hazard Monitor residue frequently of pesticides in ecology system
Acknowledgements