Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD Thomas L. Lentz, MD No part of this image collection may be distributed outside of the Yale University Intranet.

Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins, Baltimore, Kerr, J. B. Atlas of Functional Histology. Mosby, London, Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning electron microscopy. W. H. Freeman, San Francisco, Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo. Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill Livingstone, Edinburgh, Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson, Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz. Web Resources Cushing Library Educational Software/Cell Biology/Several Histology Resources Acknowledgements Sources of Micrographs, Diagrams and Figures

Bone Laboratory

Adult Bone

Some Useful Terms Compact bone = cortical bone Trabecular bone = spongy = cancellous bone Osteoid: Bone matrix not yet calcified. Woven bone: immature bone with randomly arranged collagen fibers. Produced when osteoblasts rapidly produce osteoid as in fetal bone development; fracture repair (callus). Lamellar bone: regular parallel bands of collagen. Woven bone eventually remodeled into lamellar bone. The normal form of bone in adults.

Compact bone Trabecular bone

Cortical Bone Volkmann’s canals Haversian canals Lamellar bone

Haversian canal Osteon: Lamellar Bone Osteocytes Canaliculi

Osteocytes in Lacunae in Lamellar Bone

Trabecular Bone

Trabecular Bone: XS Long Bone Muscle

Trabecular Bone Trabeculae Marrow Periosteum Endosteum

Periosteum Fibrous layer Cellular layer Compact bone Osteoclasts

Bone Marrow Endosteum

Articular Surface of Long Bone Cartilage Bone Collagen layer

Bone Formation

Intramembranous Ossification (No cartilage model; bones of skull)

Intramembranous Ossification: Fetal Skull Osteoclasts Osteoblasts

Osteoclasts Osteoblasts Intramembranous Ossification: Fetal Skull

Endochondral Ossification (Ribs, long bones, vertebrae; cartilage model)

Endochondral Bone Formation

Fetal Finger Epiphyseal growth plates (GP) Diaphysis Epiphysis (E) Epiphysis

Epiphysis With Trabecular Bone GP E Metaphysis

Growing Tibia GP E Articular cartilage M D

GP Bone Calcified cartilage Osteoid Growing Tibia

Chondrocyte Growth Sequence Resting Proliferation Hypertrophy Maturation Zone of calcifying cartilage

Development of Trabecular Bone Calcified cartilage Bone Osteoblasts; osteoid

Bone Remodeling Activation Resorption Formation Sequence

Osteoclasts Howship’s lacuna

Osteoblasts (OB) Osteoid Vessels in Resorption Canal Osteocytes in new lamellar bone

Resorption Canal XS OB Osteoid

Some pathology to think about.

Mammary Carcinoma Metastases to Vertebra

Callus at Fracture Site

New Bone at Fracture Site

Osteoporosis with Vertebral Collapse

Osteogenic Sarcoma/Osteoid