Algae from the Great Salt Plains, OK, USA: Unexpected Range of Diversity and Halotolerance Andrea Kirkwood & William Henley Dept. of Botany, Oklahoma State.

Slides:

Advertisements

Similar presentations

Characterization of nitrogenase gene distribution and activity in WCA-2A periphyton Puja Jasrotia Image source:

Advertisements

Use of the genomic data o Reconstruction of metabolic properties o Nature’s Microbiome o NGS in Population Genetics.

Chapter 50: An Introduction to Ecology & Biosphere

Isolation and Physiological Characterization of a Halophilic Host- Phage System from Solar Salterns in Baja California, Mexico or Extreme Halophiles:

Characterization of Endophytic Cellulose Degrading Fungi at the Sevilleta LTER site Zachary T. Gossage 1, Carolyn Weber 2, Cheryl Kuske 2, and Andrea Porras-Alfaro.

Salt Plains Microbial Observatory and LExEn Research: Characterization of Halotolerant Microbes William Henley 1, Kelly Major 1,4, Mark Schneegurt 2, Mark.

Eurocean 2004, Galway Ireland, slide 1 Monitoring the diversity of picoplankton in marine waters PICODIV Partners SBR CNRS, Station Biologique, BP 74,

Laboratory for Marine Microbial Ecology

© 2005 Prentice Hall Inc. / A Pearson Education Company / Upper Saddle River, New Jersey What is Metagenomics?  Traditional microbial genomics 

Problem Description: Networked Aquatic Microbial Observing System (NAMOS) Problem Description: Networked Aquatic Microbial Observing System (NAMOS) Proposed.

ACTIVITY 2: SIZE AND SCALE MATTER! Original drawings by John Tenniel.

The Ocean as a Microbial Habitat Matthew Church Marine Microplankton Ecology OCN 626/Fall 2008.

Microbial Diversity.

Brock Biology of Microorganisms

Wilson Hot Springs Using the Molecular Characterization of Microbes From Extreme Environments To Determine Microbial Diversity and Discover New Antibiotics.

Matthew D. Wallenstein Natural Resource Ecology Laboratory Colorado State University.

Using Microbial Ecology to Teach Experimental Design and Sampling Methods Mary Allen, Hartwick College Ruth A. Gyure, Western CT State University SEM of.

1.4.1 – Ecology, Ecosystem, Biosphere, Habitat & Niche

Biology 633 Environmental Plant Physiology Tropical rainforest Alpine tundra Desert Study of how the environment affects the function.

Mariya Smit and Holly Simon

Determining Microbial Assemblages in Snow Using Different Growth Media Ama Agyekum Department of Biological Sciences, York College of Pennsylvania Introduction.

Molecular Microbial Ecology

Ocean & Climate Atmospheric CO 2, DMS, … Ocean/Atmosphere Circulation Dust-Iron Influx, pH Ocean Nutrient Fields Ecosystem State Biomass Primary Productivity.

Prokaryote Taxonomy & Diversity Classification, Nomenclature & Identification Phenetic Classification Molecular Phylogeny Approach Classification (hierarchical.

Diversity of uncultured candidate division SR1 in anaerobic habitats James P. Davis Microbial & Molecular Genetics Oklahoma State University.

Monitoring of bacteria with culture-independent techniques

Barak Vasker ID Marine Biotechnology Keuter et al

Interdisciplinary Integration and Research Directions CMOP possesses a wide range of interdisciplinary research assets - Biological - Chemical - Physical.

Microbial Diversity Scott Clingenpeel. Complete Genomes 2671 Prokaryotic genomes in GenBank 114 Eukaryotic genomes – 9 Plants – 52 Animals Do we really.

Microbial Ecology Oded Beja Introduction Syllabus presentation What is microbial diversity?

GTL User Facilities Facility IV: Analysis and Modeling of Cellular Systems Jim K. Fredrickson.

1. Thousands of people live in the community shown in the photo. What are some of the other living residents? 2. What are some ways people might interact.

Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus 3 rd YEAR REVIEW SPRING FACULTY MEETING, MACON, GA SOIL MICROBIOLOGY GRIFFIN.

Culturing bioluminescent bacteria dead fish, i love you, but you’re bringing me down.

Big Picture Of ≈1.7 million species classified so far, roughly 6000 are microbes True number of microbes is obviously larger than 6000 “Imagine if our.

Florida Wetlands by:Maday & Dominique Wetlands are ecosystems typically found on the transition between terrestrial and aquatic systems.

Geographic variations in microbial cytometric diversity

Welcome Grant from National Science Foundation: Fire, Atmospheric pCO 2, and Climate as Alternative Primary Controls of C 4 -Grass Abundance: The Late-Quaternary.

Climate Effects on Species

Response of benthic algae communities to nutrient enrichment in agricultural streams: Implications for establishing nutrient criteria R.W. Black 1, P.W.

Microbial biomass and community composition of a tallgrass prairie soil subjected to simulated global warming and clipping A. Belay-Tedla, M. Elshahed,

Ecosystems. Environment and Habitat Environment Abiotic factors Biotic factors Habitat Specific place where organism is found Marine - characterized primarily.

Water 101. First organism to produce O2 (via photosynthesis) and fix N Long evolution (2.7 billion yrs) produced diverse and highly effective ecophisiological.

Influences of Changes in Salinity on Release of Carbon by Phytoplankton Jessica Wagner Century High School OGI & OHSU Lab, Field, and Modeling Study Mentors:

January 27, 2011 Summary Background on Delta Flow and Habitat Relationships Delta Stewardship Council Presentation by the Independent Consultant.

COSMOS Global Change Biology 1 July  Biodiversity What is it? How is it changing over time?  Ecosystem Functioning What is it? How is it related.

Katrina Sandona 1, Zachary Gossage 1, and Andrea Porras-Alfaro 1,2, Donald Natvig 2, Miriam Hutchinson 2 and Amy Powell 3 1. Department of Biology, Western.

Clonal diversity matters High levels of functional and genetic diversity occur in the model microzooplankter Oxyrrhis marina S chool of B iological S ciences.

1.Define a landscape. What is the focus of Landscape Ecology. Notes 2. Discuss the role of spatial and temporal scale in affecting landscape composition,

Molecular, ecophysiological and morphological data suggest that Navicula phyllepta is a species complex K. Sabbe, B. Vanelslander, V. Chepurnov, W. Vyverman.

ENVH 4387/53871 Principles of Microbial Ecology Lecture Topic Number 2 Population Dynamics ENVH 4387/5387 Biological Analysis.

September 7, 2012 Ms. Edwards. What is biodiversity? Biological =relating to living organisms Diversity = variation The variability among living organisms.

Ecology --- primary definition The scientific study of how organisms interact with the natural world.

BIOLOGY AND ALLIES SCIENCE FAIR PROJECT IDEAS. AGRICULTURE.

Soil Microbiome of Native and Invasive Marsh Grasses in Blackbird Creek, Delaware Lathadevi K.Chintapenta 1#, Gulnihal Ozbay 1#, Venu Kalavacharla 1* Figure.

Assessment of Lake Balkhash phytoplankton community structure using FlowCam imaging flow cytometer Yu-Mi Kim, Veronika Dashkova, Dmitry V Malashenkov,

BIODEEP-WP4 BIODEEP-WP5 Andrea Sass , Terry McGenity

Gilda Carvalho, Cláudia Galinha, Teresa Crespo and Maria Reis

Denaturing Gradient Gel Electrophoresis

A Comparison of Diatom Processing Methods

Biogeochemistry of Wetlands

Biology 633 Environmental Plant Physiology

Denitrification in an Urban Tidal Freshwater Wetland of the Delaware

Lab, Field, and Modeling Study

Taxonomical classification is recognizing and registering the worlds organism diversity – continual changing knowledge about evolutionary and ecological.

C.5 Freshwater phytoplankton: diversity & biology

Ecology in Middle School TEKS

Non-marine biological evidence

IBI’s: An Introduction

Microbial Diversity between Buffalo Creek and a Retention Pond

Presentation transcript:

Algae from the Great Salt Plains, OK, USA: Unexpected Range of Diversity and Halotolerance Andrea Kirkwood & William Henley Dept. of Botany, Oklahoma State Univ. Mark & Julie Buchheim Dept. of Biology, Univ. of Tulsa Other PIs: Robert Miller (OSU) & Mark Schneegurt (Wichita State Univ.)

~750 km Marine via migratory waterfowl Other Great Plains saline habitats Local soils and freshwater creeks Endemic for millenia (Permian brine) Sources of SP halotolerant microbes?

RESEARCH OBJECTIVES Henley Lab Isolate (novel) algae/cyanobacteria from SPNWR soil/water Characterize isolates phylogenetically & physiologically Relate community species composition to spatial and temporal variation in salinity and other environmental conditions Photo © 2003 NAIP aerial image database

SPNWR conditions are harsh & variable…

24 July August July 2002

SPNWR: many microhabitats in space/time How does such variability affect: - survival and physiology of individual taxa? - microbial community composition?

Some algae from SPNWR Geitlerinema Aphanothece Dunaliella Colonial! Dunaliella pennate diatoms heterocystous cyanobacterium Pico- chlorum

Example : Dunaliella Buchheim et al., in prep. SPMO endemism, adaptive radiation? MP Analysis of ITS-1 and ITS-2 data (SPMO isolates in blue)   

Kirkwood & Henley, submitted Salinity Tolerance Cyanos Diatoms Chloros  } 

Salinity-screening: Summary Note: Every soil/water sample yields <10% salinity media! Salinity (%) ? Cyanobacteria Diatoms Chlorophytes

Clone Library vs. Isolates 16S rDNA clone library sequences closely matching culture isolates (Komvophoron sp.) Numerous clone library sequences with few cultured relatives

Future Work Compare clone libraries from soil/water to our culture isolates quant-/qualitatively, and use libraries to drive isolations. Compare morphological isolates vs. 16S/18S rDNA sequences and physiology: cryptic variation? Does salinity tolerance of algal/cyanobacterial isolates match field distributions? Characterize associations between algae/cyanos and bacteria Assess spatial/temporal changes in cyano/algal community (morphological ID? DGGE? ): is it predictable? Is there a quantifiable measure of microbial stress on the flats? What really matters – salt, UV, temp., nutrients?

QUESTIONS????