Abundance and structure of microorganisms related to methane cycling in five European peatlands: Influence of plant cover and restoration stage (WP1) A.

Slides:

Advertisements

Similar presentations

The Carbon Farming Initiative and Agricultural Emissions This presentation was prepared by the University of Melbourne for the Regional Landcare Facilitator.

Advertisements

Carbon and Microbial Community Composition at Mitigated Bottomland Forest Wetlands * Elisa M. D’Angelo, A.D. Karathanasis, S.A. Ritchey, and S.W. Wehr.

Fundamentals of Soil Science

Soil Fertility and Nutrient Bioavailability Sponsored by the DEST program China Higher Education Strategic Initiatives © The University of Adelaide.

E COLOGIE ET E COPHYSIOLOGIE F ORESTIERES UMR 1137 INRA UHP.

The Carbon Farming Initiative and Agricultural Emissions This presentation was prepared by the University of Melbourne for the Regional Landcare Facilitator.

Nitrogen transformations include denitrification to N 2 O or N 2, oxidation of ammonium to nitrate, (nitrification) and anaerobic ammonia oxidation (anammox).

RECIPE - Munchen May 2005 ECOBIO. Influence of vegetation cover and age of regeneration stages on C and N soluble and microbial variables K-W test P-ValuesSOCSONSOC/SON.

Bottom-up methane dynamics along the degradation of permafrost: A case study on microbial communities of the methane cycle in a collapsing sub-Arctic palsa.

Cycling of Matter.

Carbon and Nitrogen Cycling in Soils Weathering represented processes that mainly deplete soils in elements relative to earth’s crust Biological processes.

Influence of a Wastewater Treatment Plant on Functional Characteristics of Microbial Communities Matthew S. Luckenbaugh Department of Biological Sciences,

ACTIVITY 2: SIZE AND SCALE MATTER! Original drawings by John Tenniel.

Impacts of Invasions Measuring impact is complex –What should be measured and how? −For individual plant, individual species, or multiple species? −Over.

Week 4 Lectures November 2001 Microbial Ecology and Geochemical Cycles.

Dynamics of the Northern Hardwood Ecosystem Yuqiong Hu, Jeff Plakke, Sharon Shattuck, Erin Wiley.

Ch 3 Soil Organic Matter continued.

Soil Testing Procedures for Biologically Based Systems Raymond C. Ward Ward Laboratories, Inc Kearney, Nebraska

1 Kura clover on Menfro silt loam in Chariton County, Missouri 2006 Microorganisms and Soil Health Doran et al

Roots and arbuscular mycorrhizal fungi influence nitrogen cycling in agricultural soils under contrasting management Amanda B. Daly, A. Stuart Grandy Department.

Fire Effects on Soil. What are the Functions of Soil within Ecosystems? Provides a medium for plant growth and supplies nutrients Regulates the hydrologic.

Energy and Nutrient Transfer Food chains and Food webs Objectives: *Describe a food chain and each feeding stage in it **Distinguish between a food chain.

Does Biological Diversity Control Ecosystem Function?

Revision Pack BSB IGCSE Biology.

Revision Exercises Soil. Name the three different types of parent rock? Give examples of each type of rock How is each rock type formed? What is meant.

Soil biological indicators: Organic Farming Systems Dr. Rachel Creamer, Prof. Bryan Griffiths Johnstown Castle Environment Research Centre Acknowledgements:

Recovery of Soil Functionality and Quality in a Post-Lignite Surface Mine Chronosequence in East Texas J.P. Ng, F.M. Hons, and T.J. Gentry. Texas A&M University,

Cycles of Matter Ecology Unit II.

Successional processes Hypothesis: Climate influences the rate and trajectory of succession by altering disturbance regime and the abundance of key species.

Salinity drives archaeal distribution patterns in high altitude lake sediments on Tibetan Plateau Yongqin Liu, Tandong Yao Institute of Tibetan Plateau.

Summary of the current status of the work of TUM-BO Andreas Gattinger, Matthias Weiss, Michael Schloter GSF-Research Center for Environment and Health.

WP O6 - Carbon turnover at different depths Objectives –To determine impact of recolonizing vegetation on soluble organic forms of C and N and emissions.

WP O6 - Carbon turnover Final Meeting Aberdeen 28 May - 1 June WP 6 – Carbon Turnover at different depths.

1 Mon. Tues. Wed. Thurs. Fri. Week of Oct. 6 Indoor lab – bring calculator Week of Oct. 13 No labs – Fall Break Week of Oct. 20 Wet, muddy outdoor lab.

Summary of the current status of the work of TUM-BO Scientists: Andreas Gattinger, Michael Schloter, Alexandra Hagn (DNA), Ursula Bausenwein (Socioeconomics)

Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus 3 rd YEAR REVIEW SPRING FACULTY MEETING, MACON, GA SOIL MICROBIOLOGY GRIFFIN.

Introducing ……... Slate waste. EU Life-Environment funded project: Sustainable post-industrial land restoration and re- creation of high biodiversity.

Phospholipid Fatty Acid Analysis Phospholipids are essential membrane components of all living cells Viable microbes have an intact membrane which contains.

Effects of Climate Change on Tundra Ecosystems Greg Henry, University of British Columbia Philip Wookey, University of Uppsala.

SOIL ORGANIC MATTER: Can the LTER network be leveraged to inform science and policy?

Carbon balance in a heterogeneous cutover bog in the Jura Mountains. Estelle Bortoluzzi, Daniel Epron, Daniel Gilbert, Alexandre Buttler.

Sources of nutrients to terrestrial systems

Summary of the current status of the work of TUM-BO Scientists: Andreas Gattinger, Michael Schloter Technicians: Franz Buegger (IRMS, plant labelling),

Circulation of Nutrients

Biogeochemical Cycling and Introductory Microbial Ecology

Diploma thesis (Spanien – Österreich): Title of the Project: “Effect of burning of Mediterranean macchia on ecosystem nitrogen stocks and the soil-atmosphere.

Bipolar Division.

PLFA/FAME Analyses for Microbial Community Assessment

BIOGEOCHEMISTRY. What is Biogeochemistry? The study of the biological, geological and chemical factors that influence the movement of chemical elements.

Environmental Microbiology (MLEM-201) What do Microbes do? How can we use this to our advantage? Lecturer: Dr. Mohamed Salah El-Din.

Earth, Ecological, & Environmental Sciences

Δ 13 C Variation from Plants to Soil Jonathan Harris MEA 760 NCSU.

Comparison of Soils and Plants at Prairie Ridge: % C and % N Lori Skidmore.

ECOSYSTEM ECOLOGY … the integrated study of biotic and abiotic components of ecosystems and their interactions. To achieve this integration: follow the.

ECOSYSTEMS All of the organisms living in a community and the abiotic factors with which they interact. “global ecosystem” Energy flows Nutrients cycle.

Maria Bañuelos University of Redlands University of Oregon

Influence of Nitrogen Sources and Soil pH on Soil Microbial Communities in a Long-term Crop Rotation System Reji Mathew, Yucheng Feng, and Charles Mitchell.

Actinomycetes and Filamentous Bacteria

Microbial Community Analysis in Monosodium-methanearsonate Treated Rice Soils Anil Somenahally 1, Terry Gentry 1, Richard Loeppert 1 and Wengui Yan 2 1.

Compost pH. pH pH decreases as the [H+] increases pH range is 0-14 At 25C the pH of an acidic solution is less than 7.00 At 25C the pH of a basic solution.

Gross nitrogen mineralization and fungi-to-bacteria ratios are negatively correlated in boreal forests Mona N. Högberg, Yu Chen, Peter Högberg; Biol Fertil.

Mechanistic modeling of microbial interactions at pore to profile scales resolve methane emission dynamics from permafrost soil Ali Ebrahimi and Dani Or.

Department of Agronomy

Biogeochemistry of Wetlands

ASSESSING AND MANAGING SOIL QUALITY FOR SUSTAINABLE

Chemical Properties of Forest Soils in the Catskills Region

Global changes alter soil fungal communities

Nitrogen Carbon water 7th science Malnory/Pietsch.

Relevant Biomarkers of Inflammation and Their Possible Role in Atherosclerosis Arpita Basu, et al. ATVB, 2006; 26;

Zhen,Y.,Sara, K.,Tianran,S.,Ruben, K. and Andreas,K.

Presentation transcript:

Abundance and structure of microorganisms related to methane cycling in five European peatlands: Influence of plant cover and restoration stage (WP1) A. Gattinger et al. Technical University of Munich (at the campus of GSF-Research Center for Environment & Health) Chair of Soil Ecology D Neuherberg

I. Depth distribution of archaeal (methanogenic) biomass among countries Country x Depth i20:0 I20:1 I40:0 i40:1cyx i40:1cy i40:2cyx i40:2cy Finland France/B France/LScotland Switzerland 0,00 20,00 40,00 60,00 80,00 100,00 PLEL_(nmol/g dm) 329 Depth Finland France/B France/LScotland Switzerland Pooled for „country“

I. Depth distribution of methanotrophic biomass (Type I and II) among countries Finland France/B France/L ScotlandSwitzerland PLFA (nmol/g dry matter) Country x Depth 0,00 20,00 40,00 60,00 80,00 100,00 120,00 140,00 Depth Finland France/B France/LScotland Switzerland Country 0,00 5,00 10,00 15,00 Type I Type II Finland France/B France/LScotland Switzerland 15,00 Pooled for „country“ Country x Depth

II. Methanogens to methanotrophs along gradients Finnland Jura sites

…the presented data should be combined with CH4/CO2 flux data for making system-related studies. ………………………………………………………………………………….. PLFA analyses completed (WP1) apart from specific biomarkers, data of other PLFA is avavailable (saturated, mono-unsaturated and polyunsaturated fatty acids) for calculating total biomasses of eg. Bacteria, fungi, etc.

Labelled litter 13 C - 15 N 15 N mineralization towards microbes Microbial communities : 13 C PLFA analysis 13 C & 15 N in microbial biomass towards peat 13 C & 15 N (K 2 SO 4 extract without fumigation) Peat column WP3: Microbial transformations of plant litter (TUM-BO, ECOBIO, EPFL/WSL) (TUM-BO, ECOBIO, EPFL/WSL)

What do we want to know? 1.Carbon transformation  How much plant C is consumed by the microbial biomass?  How much plant C is used by Bacteria, Archaea, Eukarya and when?  How much plant C is „somehow“ stabilised? 2. N transformation  How much plant N is consumed by the microbial biomass?  How much plant N is mineralised?  How much plant N is somehow stabilised? Labelled litter 13 C - 15 N 15 N mineralization towards microbes Microbial communities : 13 C PLFA analysis 13 C & 15 N in microbial biomass towards peat 13 C & 15 N (K 2 SO 4 extract without fumigation) Peat column

Simultaneous identification and quantification of PLFA/PLEL from environmental samples and their corresponding 12 C/ 13 C ratios by GC/MS-c-IRMS MS (DSQ) IRMS (DeltaPlus Advantage ) 20% of the analyte 80% of the analyte Agilent MSD

SATFAs (Bacteria) PUFAs (Eukarya)

Bacteria: Gram-positive (i15:0) ControlSphagnumEriophorum δ 13C (‰) sampling date (= 15d, 60d, 150d) Horizon 012

Bacteria: Gram-positive (a15:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

Bacteria: Gram-negative (cy17:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

Bacteria: Gram-negative (cy19:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

Archaea: Euryarchaeota (i20:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

Eukarya: Fungi (18:2d9,12) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

Eukarya: Protozoa (20:4d5,8,11,14) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

What needs to be done? 1.Carbon transformations  LC/IRMS of prepared microbial biomass C (CFE) extracts  Calculation of group-specific microbial plant C utilisation  Mass balance for the „whole“ system 2. Nitrogen transformations  EA-IRMS of prepared microbial biomass N (CFE) extracts  EA-IRMS of mineral N extracts (?)  Calculation of microbial plant N utilisation  Mass balance for the whole system

LC IsoLink

Chromatogramm Standard

Reproduzierbarkeit des LC/IRMS-Signals δ 13 C [‰ PDB] n = 3 + SE Neue Methode ist:  reproduzierbar stabil  sensitiv  effizient (ca. 100 Proben/Tag)