Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha
Fertilization – transformation of meiotic cells into mitotic cells Gametes – terminally differentiated and highly specialized Genomes - (oocyte, sperm) transcriptionally inactive after fertilization One cell stage embryo is totipotent Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES
A)Sheep B)Mouse C)Human D)Rabbit E)Bovine F) Pig G) Monkey
Mouse embryo Human embryo PB MPN FPN MPN 5-MeC
Human embryo
PB PN MPN FPN 49% of human 1-cell stage embryos showed abnormal demethylation pattern
2PB FP MP
Wilmut, I et al. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385,
A B AB
Enukleace
Přenos jádra
What do we need??
Oct4Cdx2 DAPI merge
Nanog
Oct4DAPI
Differentiation βIII tubulin TROMA 1 DAPI MF20 + EB formation
Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň
Pacient ESc linie
Producing primate embryonic stem cells by somatic cell nuclear transfer Nature - doi: /nature06357 …. potřebujeme vůbec přenosy jader?
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