Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha Fertilization – transformation of meiotic cells into mitotic cells  Gametes – terminally differentiated.

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Presentation transcript:

Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha

Fertilization – transformation of meiotic cells into mitotic cells  Gametes – terminally differentiated and highly specialized  Genomes - (oocyte, sperm) transcriptionally inactive after fertilization One cell stage embryo is totipotent Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES

A)Sheep B)Mouse C)Human D)Rabbit E)Bovine F) Pig G) Monkey

Mouse embryo Human embryo PB MPN FPN MPN 5-MeC

Human embryo

PB PN MPN FPN 49% of human 1-cell stage embryos showed abnormal demethylation pattern

2PB FP MP

Wilmut, I et al. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385,

A B AB

Enukleace

Přenos jádra

What do we need??

Oct4Cdx2 DAPI merge

Nanog

Oct4DAPI

Differentiation βIII tubulin TROMA 1 DAPI MF20 + EB formation

Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň

Pacient ESc linie

Producing primate embryonic stem cells by somatic cell nuclear transfer Nature - doi: /nature06357 …. potřebujeme vůbec přenosy jader?

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