ABTECH Scientific, Inc. Virginia Biotechnology Research Park 800 East Leigh Street, Suite 52 Richmond, Virginia USA Microlithographically Fabricated Chips and Microelectrodes for Chemical and Biological Sensor Research and Development

Microfabricated Interdigitated Microsensor Electrodes (IMEs) IME XX50.5 M, CD, FD; Au, Pt, ITO XX = 5, 10, 15, 20  m

Microelectrodes Oxygen Microelectrodes pH and REF Microelectrodes

IME FD Au, Pt, ITO Liquid Flow Cell Detector

IME FD Au, Pt, ITO Gas / Vapor Flow Cell Detector

MDEA XXX Au, Pt Microdisc Electrode Arrays 1

MDEA DEVICES Disc dimensions / Number of Discs Active Area Conductor MDEA 3600 D = 3,600  m 1 disc 0.10 cm 2 Au, Pt, ITO MDEA 250 D = 250  m 207 microdiscs 0.10 cm 2 Au, Pt, ITO MDEA 100 D = 100  m 1,296 microdiscs 0.10 cm 2 Au, Pt, ITO MDEA 50 D = 50  m 5,184 microdiscs 0.10 cm 2 Au, Pt, ITO 50  m100  m250  m x  m x 50 Microdisc Electrode Arrays 2

50  m 100  m 250  m 3600  m Microdisc Electrode Arrays 3

PAnCl on MDEA 50 Au x50 PAnCl on MDEA 100 Au x50 PAnPSS on MDEA 100 Au x50 PAnPSS on MDEA 50 Au x50  Total current density applied = 100 mA/cm 2  Total time = 20 s  Total charge density = 2 C/cm 2 Microdisc Electrode Arrays 4

Independently Addressable Microband Electrodes (IAMEs) XX = 5, 10, 15, 20  m Microband Electrodes IAME XX04, Au, Pt or ITO

Independently Addressable Interdigitated Microsensor Electrodes (IAIMEs) XX = 5, 10, 15, 20  m Microband Electrodes IAIME XX10, Au, Pt or ITO

A B C D E F G Electrochemical Cell-on-a-Chip (ECC)

XX = 05, 10, 15, 20  m ECC IME XX04, Au, Pt or ITO 2 cm 1 cm C1 C2 A B C D E F G

Counter Electrode Interdigitated WE1 and WE2 Reference Electrode, Ag/AgCl Si 3 N 4 Passivation Layer Unmodified Polypyrrole modified Unsilverized Silverized Electrochemical Cell-on-a-Chip (ECC)

An integrated biochip showing the microfabricated pattern of electrodes bonded and sealed into the Microncs’ microfluidic T-cassette. Electrochemical Cell-on-a-Chip (ECC) Integrated in a Microfluidic Cassette Polypyrrole modified

IAME-co-IME 2-1 Au, Pt Independently Addressable Microband Electrodes and Microfrabricated Interdigitated Microsensor Electrodes)

4 bands 3 mm long 2 m lines, 1 m space fingers 3 mm long 2 m lines, 1 m space IAME-co-IME 2-1 Au, Pt Independently Addressable Microband Electrodes and Microfrabricated Interdigitated Microsensor Electrodes)

IAME-co-IME 2-1 Au, Pt

Independently Addressable Microband Electrodes and Microfrabricated Interdigitated Microsensor Electrodes) 2  line 1  space

Chips of 5μm, 10μm, 15μm and 20μm with electropolymerized PPyCSA Sensor Arrays of VOC Responsive Polymers for the Electronic NOSE

People Scientific Advisory Board Anthony Guiseppi-Elie, Sc.D. David Allara, Ph.D. (Penn State University) Norman Sheppard, Jr. Management Team James W. Sharpe, MBA Chairman and CEO Anthony Guiseppi-Elie, Sc.D. President and Scientific Director James R. Funkey, MA, CPA VP and Controller Ann M. Wilson Director, Applications Development TBH VP, Business Development Board of Directors James W. Sharpe, MBA Chairman Anthony Guiseppi-Elie, Sc.D. President and Scientific Director Annette Guiseppi-Elie, Ph.D. Cynthia Garwon-Burke, Ph.D. Patent and IP Council Leigh P. Gregory Patent Attorney Clemson, SC, USA