In the name of GOD

Molecular imprinted polymer (MIP) Supervisor Dr. Parchehbaf Presenter Meysam abdi 1391/09/ /12/05

Content  Introduction  History  Principles  Advantage  Application  Conclusion 3

Introduction Base of the method, is recording of target molecule structure In Polymer memory at polymerization process. so that the identification, synthesized polymer only detects the target molecule. 4

History  1894, Fischer lock & key theory.  1930, Polyakov, molecular imprinting in silica’s matrix  1949, Linus pauling theory, change of antibody structures  1977, Gunter wulff, molecular imprinting by using covalence banding  1981, Klaus mosbach, molecular imprinting by using non-covalence banding  1995, Witcombe, molecular imprinting by using semi-covalence (hybrid) banding 5

Research procedure 6 C. Alexander, L. Davidson, and W. Hayes, Tetrahedron 59, (2003).

Principles 7

Fischer Lock & Key theory 8 Monomer & cross linker Template molecule bonding Remove template molecule PowerPoint of J.jafari thesis

Variety methods of MIP production 9

Advantage  high selectivity  high pre-Concentration factor  the sorbent is reusable for many times 10

Application of MIP 11 MIP Sensor Pre- concentrati on PharmacyCatalysesSeparation Selective sorption Purification

Sensor 12 T. Alizadeh et al. / Electrochimica Acta 55 (2010) 1568–1574

Caffeine (1,3,7-trimethylxanthine) is an alkaloid 13 Caffeine Fountain Tea kola nuts Cacao beans Coffee

MIP-CP & NIP-CP responses 14

MIP-CP electrode selectivity 15

Real sample analysis 16

17 T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

Electrode responses & washing effect 18

Selection of elecrochemical methods 19

Analytical characterization 20

21 T. Alizadeh et al. / Analytica Chimica Acta 689 (2011) 52–59

Hg 2+ Properties Soluble in water High reactive toxic Extreme Volatility living tissues 22 Hg 2+ can in turn cause symptoms paranoia Sleep lessness fever memory loss weaknesstremors

Comparison of the IIP-CP & NIP-CP electrodes 23

Evaluation the selectivity of sensor 24

Determination of Hg 2+ in real samples 25

26 T. Alizadeh, S. Amjadi / Journal of Hazardous Materials 190 (2011) 451–459

27 Pb 2+ importance toxicological Effects interferes with calcium Poisoner environmental pollutant enzyme inhibitor Found in Soil AirWater

Comparison of the prepared electrodes 28

Evaluation of the selectivity of IIP-CP electrode 29

Evaluation of the effect of extraction conditions 30

Determination of Pb 2+ in real samples by proposed sensor 31 T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

Prepared electrode 32 T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

a new electrochemical sensor for determination of compounds at trace levels was introduced. Application of polymer as a novel modifying agent in the carbon paste electrode made it very selective for compounds determination in the presence of common potential interfering agents. The polymer, used in the carbon paste composition, acted as the selective chemical interface of the sensor as well as a pre-concentration agent. 33 Conclusion

Thanks for your attention template of PowerPoint from: