Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase.

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Presentation transcript:

Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase

Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink

Gram Reaction Morphology O2Ferment Lactose Urease E. coli (-) Bacilli Facultative (+) (-) Proteus vulgaris (-) Bacilli Facultative (-) (+) Salmonella (-) Bacilli Facultative (-) Shigella (-) Bacilli Facultative (-)

Urease Test

Gelatinase Gelatin (solid) Gelatin (liquid) Tryptic Soy Gelatin Agar

Gelatinase Test

Nitrate Reductase Nitrate Nitrite + water Facultative Bacteria Nitrate Broth