1 Introgression mapping in the grasses Lolium perenne and Festuca pratensis Julie King
2 LpLpLpLp x FpFp (28) (14) LpLpFp x LpLp (21) (14) BC 1 (14) Production of substitution lines
3
4 Chromosome 1 Chromosome 2 Chromosome 3 Chromosome 4 Chromosome 5 Chromosome 6 Chromosome 7
5 LpLpLpLp x FpFp (28) (14) LpLpFp x LpLp (21) (14) LpLp(Fp) x LpLp (13Lp+1Fp) (14) LpLp(Fp) (14) BC 1 BC 2 BC 2 introgression population
6 Lolium 2n=2x=14 7 Lolium/Festuca substitutions Lolium/Festuca substitution lines
7 Introgression chromosome series X
8 Genetic map of chromosome 3
9
/26 3 3/ /3 3/ / BC 2 plants used for physical mapping
11
12 RZ783, C112 C949 CDO345 C250, C122, WG889, BCD128 CDO455 R1928, PSR370, RZ382, PSR394, CDO920 R1613 CDO328 CDO466, C30
13 Rice chromosome 1: BAC No. BAC/PAC BAC/PAC coordinates 1 P0672D08 1 – 173,729 3 P0005A05 300,777 – 436,798 8 P0463F06 851,331 – 919, P0037C04 1,345,101 – 1,437, P0408F06 1,566,828 – 1,698, P0024G09 2,381,624 – 2,444, P0009G03 2,715,057 – 2,855,651…
14 BAC 227 (PAC clone PO686E09) Festuca AATTCTGGTTCATGTGTTCCTGTCGACATTGAGTGGCCTCCTAGAAGAGCA Lolium (4n) AATTCTGGTTCATGTGTTCCTGTCGACATTGAGTGGCCTCCTAGAAGAGCA Lolium (2n) AATTCTGGTTCATGTGTTCCTGTCGACATTGAGTGGCCTCCTAGAAGAGCA CATCATCTGAACTTTAATGCACCATTTTCTATGAACATGGATACTAAATCTG GAGAGCTGTCAAATGACGGACTCAATGATGTCGATCCTTTTGCCGATTGGC GAGAGCTGTCAAATGACGGACTCAATGATGTTGATCCTTTTGCCGATTGGC
15 BAC mapping discrepancies BACSubline
16
17, NOR. Centromere Genetic Distance cM 0 Physical distance % Genotype 11 3/26 6 3 3/10 36 17 83 92 3/2 99 83 19 56 3/23 2/3 18 % BACs 82% BACs 9% BACs 15.5% 64.5% 20%
18
19, NOR. Centromere Genetic Distance cM 0 Physical distance % Genotype 11 3/26 6 3 3/10 36 17 83 92 3/2 99 83 19 56 3/23 2/3 18 % recombination = very low 50% BACs 47% genes 53% = T + H
20 Determining genetic control of target traits Locate gene(s) controlling trait to chromosome(s) using substitution lines. Locate gene(s) controlling trait to a particular bin using physical mapping population. Use bin mapped rice BACs to exploit rice genome for further markers /candidate genes. Springboard for gene isolation.
21 Nutrient use efficiency High nitrogen uptake - chr. 3 High nitrogen utilisation - chr. 4
22 All involved: Ian King Ian Armstead Iain Donnison Luned Roberts John Harper Kirsten Skot Lin Huang