InfraCal TOG/TPH Analyzer, Model CVH Extraction Procedure

The sample extraction can be done using either a prescription bottle with a septa cap or a separatory funnel

Extraction using a prescription bottle with a septa cap

Collect a measured amount of sample Collect a measured amount of sample

For samples that will be stored for more than a day or for samples with a high pH: Adjust the pH to less than 2 with Hydrochloric acid or Sulfuric Acid (typically 3-5 drops depending on buffers in sample)

Add Solvent Add Solvent 10 parts sample to 1 part solvent For example: For 150 ml of sample add 15 ml of solvent

Shake for 2 minutes Shake for 2 minutes

Open the cap on the bottle during the 2 minute shaking to relieve the pressure Open the cap on the bottle during the 2 minute shaking to relieve the pressure

Solvent is heavier than water and will sink

Turn bottle upside down Turn bottle upside down

Use the syringe to remove the solvent Use the syringe to remove the solvent

Fill the cuvette with solvent Fill the cuvette with solvent

Place the cuvette in the InfraCal frosted side to the front Place the cuvette in the InfraCal frosted side to the front

Press the run button Press the run button

Oil concentration displayed Oil concentration displayed 14

Extraction using a separatory funnel

Pour measured sample into separatory funnel Pour measured sample into separatory funnel

Measure one tenth of the sample size of solvent Measure one tenth of the sample size of solvent For example: For 150 ml of sample add 15 ml of solvent

Pour the solvent into the sample collection container to rinse interior surfaces and cap of left over oil Pour the solvent into the sample collection container to rinse interior surfaces and cap of left over oil

Pour the solvent from the sample container into separatory funnel containing sample Pour the solvent from the sample container into separatory funnel containing sample

Shake for 2 minutes Shake for 2 minutes

Stop to vent during the 2 minutes shaking to relieve the pressure Stop to vent during the 2 minutes shaking to relieve the pressure

Put the separatory funnel into the stand Put the separatory funnel into the stand

Allow the solvent to sink to the bottom Allow the solvent to sink to the bottom

Fill the cuvette with solvent Fill the cuvette with solvent

Place the cuvette in the InfraCal frosted side to the front Place the cuvette in the InfraCal frosted side to the front

Press the run button Press the run button

Oil concentration displayed Oil concentration displayed 14

Silica Gel Extraction The difference between TPH (Total Petroleum Hydrocarbon) and TOG (Total Oil and Grease) is the polar organics are removed from the extract using silica gel. The remaining hydrocarbons are the non-polar components considered to be TPH

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel Put solvent extract into cuvette Put solvent extract into cuvette

Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel Put solvent extract into cuvette Put solvent extract into cuvette Put cuvette into InfraCal frosted side to the front Put cuvette into InfraCal frosted side to the front

Press the run button Press the run button

Oil concentration displayed Oil concentration displayed 14

For questions please contact us at: or InfraCal TOG/TPH Analyzer, Model CVH Extraction Procedure