Hematopoeisis VIBS 443/602

Peripheral blood smear (May-Grunwald-Giemsa) 113 Peripheral blood smear (May-Grunwald-Giemsa)

110 Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils

Bone marrow 19761 19761

Bone marrow smear; low power view, showing negative images of fat globules

Megakaryocyte: contain many joined nuclei Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA Nuclei of megakaryocyte Red blood cell

Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation. Macrophage Lymphocyte Neutrophil

Pronormoblast Basophilic normoblast Polychromatophilic normoblast Orthochromatic normoblast Myelocyte Late metamyelocytes

Pronormoblast Basophilic normoblast Polychromatophilic normoblast Orthochromatic normoblast Myelocyte Late metamyelocytes

Late promyelocyte Late myelocyte/early metamyelocyte Nearly mature PMN

Smudge cell Promyelocyte Myelocyte Metamyelocytes

Pronormoblast Metamyelocytes Band cell

Bone marrow: EM 12a Reticular cell Developing red blood cell

Bone marrow; EM 8a Lymphocyte PNM Red blood cell eosinophil

Bone marrow; EM 12b PMN Immature neutrophil Red blood cell

Routine H&E of bone Marrow spicules Lipid in fat cells

H&E of bone marrow 1. megakaryocytes

19761

H&E of bone marrow: Red blood cell

Rib 420

420 Rib

421 Tibia, fetal

32583

32583

Red bone marrow smear (May-Grunwald-Giemsa) 112 Red bone marrow smear (May-Grunwald-Giemsa)

Cells of Erythropoisesis   Pronormoblast round nucleus and thin rim of deep blue cytoplasm Basophilic normoblast nuclear condensation ("fractured nucleus") with a deep blue cytoplasm

Polychromatophilic normoblast   Polychromatophilic normoblast cytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red Orthochromatic normoblast must have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed

Reticulocyte no nucleus, mature cytoplasm with blue tint Red blood cell typical of mature cell seen in circulation

Cells of Granulopoiesis Promyelocyte oval nucleus with deep blue cytoplasm and azurophilic granules   Myelocyte cytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape

Metamyelocyte deeply indented nucleus with nearly mature cytoplasm (no longer blue) Band cell nucleus just prior to lobulation with fully mature cytoplasm Neutrophil (mature) lobulated nucleus with fully mature cytoplasm