Hematopoiesis Simplified: Part 1 Erythropoiesis Larry Johnson Texas A&M University

Hematopoiesis Simplified: Part 1 Erythropoiesis Objectives are to: Identify the developmental cells of erythropoiesis Characterize developmental changes in the cytoplasm and nucleus of these cells Bone marrow 32583 Blood vessel

19761 Bone marrow Blood in vessel

Peripheral blood 21 Erythrocytes Reticulocytes

Erythropoiesis Erythrocytes No nucleus and red cytoplasm Reticulocytes No nucleus and blue - grey cytoplasm Orthochromatophilic erythroblasts Dark, small, spherical nucleus blue – grey cytoplasm Darkening, fractured ,spherical nucleus and mixed pools of grey and blue cytoplasm Polychromatophilic erythroblasts Fractured, spherical nucleus and thin rim of dark blue cytoplasm Basophilic erythroblasts Uniformly light, spherical nucleus with thin rim of medium to dark blue cytoplasm Proerythroblasts

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Erythropoiesis Proerythroblasts Erythrocytes Reticulocytes Orthochromatophilic erythroblasts Basophilic erythroblasts Polychromatophilic erythroblasts Erythropoiesis

Orthochromatophilic erythroblasts Erythrocytes

Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

Polychromatophilic erythroblasts

Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

Basophilic erythroblast

Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

Proerythroblasts

Erythropoiesis Orthochromatophilic erythroblasts Erythrocytes Reticulocytes

Early orthochromatophilic erythroblasts Late polychromatophilic erythroblasts

Polychromatophilic erythroblasts Early polychromatophilic erythroblasts

Basophilic erythroblasts Proerythroblasts

ut 112 Orthochromatophilic erythroblasts RBCs Late polychromatophilic erythroblast Basophilic erythroblast platelets Polychromatophilic erythroblast Reticulocyte Eosinophil or basophil Erythrocytes Proerythroblast

Bone marrow Reticulocyte entering blood vessel Blood vessel 32583

The End of Hematopoiesis Simplified: Part 1 Erythropoiesis

Granulopoiesis is next

Hematopoiesis Simplified: Part 2 Granulopoiesis Larry Johnson Texas A&M University

Hematopoiesis Simplified: Part 2 Granulopoiesis Objectives are to: Identify the developmental cells of granulopoiesis Characterize developmental changes in the cytoplasm and nucleus of these cells 32583 Bone marrow

19761 Bone marrow Blood Blood

` 32583 Bone marrow Developing granulocytes

Peripheral blood 21

Granulopoiesis Lobulated, dark nucleus, mature grey cytoplasm with small granules Neutrophils Neutrophilic band or stab cells Horse-shoe shaped, darkening nucleus, grey mature cytoplasm Neutrophilic metamyelocytes Oval to kidney bean-shaped, indented nucleus, grey mature cytoplasm with a weak blue tent Large, oval, non-indented nucleus, large amount of cytoplasm with specific and non-specific granules Neutrophilic myelocytes Large, oval, non-indented nucleus, large amount of sky blue cytoplasm with non-specific granules Promyelocytes

Granulopoiesis Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic metamyelocytes

ut 112 Neutrophil Neutrophilic metamyelocytes Neutrophilic metamyelocyte Platelets Eosinophil or basophil Neutrophilic myelocytes

Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilic metamyelocytes Granulopoiesis

Neutrophilic metamyelocytes Lymphocytes Neutrophilic myelocytes Unknown nuclei Promyelocyte Neutrophilic band cells Lymphocytes Neutrophilic myelocytes Neutrophilic band cells Neutrophilic metamyelocytes Neutrophilic band cell

Neutrophilic band cells Neutrophils Granulopoiesis

Neutrophilic metamyelocytes Early neutrophilic metamyelocytes

Neutrophilic myelocytes

Late promyelocytes Promyelocyte

Neutrophilic metamyelocytes Unknown nuclei Neutrophilic myelocytes Neutrophilic band cells Neutrophilic metamyelocytes Proerythroblast Orthochromatophilic erythroblasts Orthochromatophilic erythroblasts Eosinophils or basophils

Bone marrow White blood cell entering a blood vessel 32583 Blood

The End of Hematopoiesis Simplified: Part 2 Granulopoiesis