Concentrated Algae formulations Paste, Dried Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Algae paste Low density rotifer culture High density rotifer culture Green water Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Algae Paste Algae Pastes are a super-concentrated liquid microalgae feed for larval fish, shrimp and bivalve shell fish. Pastes are used to supplement or replace live algae grown on-site at the hatchery. It is made from microalgae grown intensively in large industrial facilities but concentrated by removing most of the water. It is not live (reproducing) but will remain in the water column similar to live algae Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Advantages · Reduce risks - Use it as a primary feed, or as backup in case of an algae culture crash. · Reduce overhead and production costs - Less electricity, less space, fewer technicians. · Reduce live food production space - live algae systems need a lot of space that could be used for growing your target animals. · Easy to Use liquid concentrate - use only the amount of algae you need at each feeding. · Relatively long Shelf Life - 8 to 12 weeks in the refrigerator (depending on the culture). Nannochloropsis and Tetraselmis can be frozen for long life. Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Production method Algae Paste is a slurry of concentrated micro algae cells. The algae is cultured by the batch system in mono-specific cultures. These batches are continuously monitored for quality control, and when they are in log growth phase and are at their nutritional peak they are concentrated by high volume, continuous flow centrifuges. This concentrate, or Algae Paste then has a food grade preservative added to it that provide a relatively long shelf-life. Live food aquaculture training course www.aquatrain.org

Large scale production - tanks Live food aquaculture training course www.aquatrain.org

Large scale production - ponds Live food aquaculture training course www.aquatrain.org

Large scale production - bioreactors Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Centifuge Cream skimmers, centrifuges, etc Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Species available Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Characteristics Live food aquaculture training course www.aquatrain.org

Nutritional characteristics Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course HUFA Characteristics Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Microalgae Ice Cubes Algae paste can be frozen into small ice cubes creating a pre-measured quantity of algae extends the shelf life of the algae. When frozen, break the cubes out of the trays and store them in a plastic bag in the freezer. The cubes can be added directly to the tank or dissolved in a jar with water from the tank and then added to the tank. Live food aquaculture training course www.aquatrain.org

Gravity Flow Method (Simple and inexpensive) Hang the bucket slightly above tank  Use a thin plastic tube to siphon the algae from the bucket into the tank  Use a clip to regulate the flow of algae so it will run for a full day at a constant drip In warm environments add a frozen "gel ice" to the bucket to keep the algae concentrate cool Live food aquaculture training course www.aquatrain.org

Metering Pump (Better control) Use an inexpensive metering or dosing pump ($150-200) to maintain constant flow rate The bucket can be placed in a refrigerator or can be a purpose made refrigerated tank located anywhere in the hatchery and can feed chosen tanks at the same time Live food aquaculture training course www.aquatrain.org

Spray-dried Schizochytrium sp.AlgaMac-2000 spray-dried heterotrophic strain of microalgae, Schizochytrium sp., rich in both n-3 and n-6 long chain fatty acids Schizochytrium sp. (American Type Culture Collection 20888) biomass was produced in a 400-L fermenter At the end of the fermentation, the cells were concentrated by centrifugation, spray-dried, and vacuum sealed in foil packets. Live food aquaculture training course www.aquatrain.org

Heterotrophic Production Species of heterotrophic microalgae that could be grown in conventional fermentation systems might be produced at a much lower cost than microalgae produced in outdoor ponds. Production controls inherent in fermentation systems have the potential to facilitate production of heterotrophic algae with an improved and more consistent biochemical quality. Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Algamac Products Previous attempts have been made to utilize spray-dried heterotrophic microalgae as aquaculture feeds. The strains chosen, however, were selected primarily for their heterotrophic mass production attributes, with only a secondary concern for their nutritional profile, especially in terms of their n-3 and n-6 HUFAs. As a result, they generally performed poorly as feed for larval marine organisms Live food aquaculture training course www.aquatrain.org

Physical characteristics Particle size analysis indicated a range from 3-18 um with an average particle size of 7.5 um Suspension in water Approximately 50% of the cells remained suspended after 6 h of static conditions (no mixing or aeration) in all three treatments Live food aquaculture training course www.aquatrain.org

Proximate analysis and fatty acid profile Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Rotifer enrichment Rotifers fed Schizochytrium sp. for only 8 h had DHA, EPA, and arachidonic acid contents of 18.3%, 0.3% and 1.4% of total fatty acids Rotifers fed Schizochytrium sp. for 24 h have DHA, EPA, and arachidonic acid contents 17.7%, 5.7% and 6.5% of total fatty acids respectively. Rotifers fed Schizochytrium sp. for 24 h do not exhibit further increased concentrations of DHA in their fatty acids but do exhibit increased EPA, and arachidonic acid concentrations Live food aquaculture training course www.aquatrain.org

Live food aquaculture training course Artemia enrichment Nauplii enriched with Schizochytrium sp. for 24 h had an EPA content of 0.4% of dry weight and a DHA content of 0.1%. The highest enrichment level occurred at 400 mg/mL of Schizochytrium. The EPA content in the nauplii was 0.5% of dry weight and the DHA content was 0.8%. Live food aquaculture training course www.aquatrain.org