Bio & 241 A&P 1 Unit 3 / Lab 4.

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Bio & 241 A&P 1 Unit 3 / Lab 4

Histology Slides for Muscle Tissues Slides are presented in order of magnification if different view are presented. As you view the following slides, make sure you can accomplish these goals: Can you identify the tissue observable on the slides? Can you identify the specific structures or layers indicated by the numbered arrows or brackets? At the end of a sequence, you will find the answers to the above for each slide.

Type of Muscle Tissue? 1 3 2 1 400X

Type of Muscle Tissue? 1 2 4 400X

Type of Muscle Tissue? 6 5 5 7 400X

8

Type of Muscle Tissue? 9 10 100X

Type of Muscle Tissue? 9 10 11 400X

Answers to Slides 2 through 7 Slide 2: Skeletal Muscle showing both longitudinal and transverse views Slide 3: Skeletal Muscle in longitudinal view Slide 4: Cardiac Muscle in longitudinal view Slide 5: Individual smooth muscle cell Slide 6: Transverse section of the GI tract showing muscle layers Slide 7: High power view of transverse section of the GI tract showing muscle layers Numbered arrows: Peripheral nuclei of skeletal muscle cells. Note multiple nuclei associated with each cell A and I bands of a skeletal muscle fiber Blood vessel in the perimysium Perimysium between muscle fascicles Intercalated discs Branching fibers

Answers to Slides 2 through 7 Centrally located nucleus Isolated single smooth muscle cells with nucleus Longitudinal smooth muscle layer of GI tract wall Circular smooth muscle layer of the GI tract wall Spindle-shaped smooth muscle cells

100X 14 13 15 12 16 14

400X 15 13 15

100X 18 17 19 20 20 13

400X 18 20 2

Answers to Slides 10 through 13 Slide 10: Muscle-Tendon intersection low power Slide 11: Muscle-Tendon intersection high power Slide 12: Neuromuscular junction low power Slide 13: Neuromuscular junction high power Numbered arrows: 12. Skeletal Muscle Skeletal muscle cell Epimysium surrounding muscle Endomysium joining the tendon Tendon Portion of a Motor unit of a muscle Motor nerve Axon from a motor nerve Neuromuscular junction with Ach vesicles

3 5 3 4 1 2 5 6

5 6 7 7

Answers to slides 15 through 13 Slide 15-16: Nerve artery vein slide with Masson stain for connective tissue Numbered Arrows: Transverse section through a nerve Vein Transverse section through a muscle fascicle Epimysium covering the muscle Perimysium surrounding a muscle fascicle Adipose Tissue Endomysium surrounding muscle fibers