Title / Innovation Product Group Members name 1 Universiti Kuala Lumpur Malaysian Institute of Chemical & Bioengineering Technology (UniKL MICET) Section of Chemical Engineering Technology, Alor Gajah, Melaka, Malaysia. xxxxxx Introduction Experimental / Processes Figure 1 Figure 2Figure 3 ABAB Result / End Product Benefits of the Products End Product Materials End Product Natural rubber latex (NRL) has been one of the most important raw materials in the manufacturing of rubber related products used for medical, dental care, as well as household needs. In the past few decades, latex allergy has been diagnosed as a considerable risk factor for severe generalized allergic reactions and hence attracted attention worldwide. One of the allergen content in latex products is protein that remains in manufactured natural rubber latex products. The aim of the present study is to deproteinize the latex protein content in natural rubber latex so as to reduce the amount of allergens content in natural rubber products by enzymatic treatment. The proteolytic enzyme used in the study is protease from Basillus Sp. The quantitative study of protease and the characteristic effect of different types of surfactants on the deproteinization process were investigated. Five different surfactants used were sodium acetate, sodium thiosulphate, sodium thiosulphate pentahydrate, sodium hydrogen carbonate and sodium lauryl sulphate. The degree of deproteinization was measured in terms of nitrogen content using Kjeldhal method. 1. Preparation of Latex Solution 2. Deproteinization Treatment 2.1 Seven solutions were prepared by using different surfactants and amount of enzyme as shown in the Table 1. Table 1 : Samples for Proteolytic Enzyme Treatment SampleProtelolytic Treatment: Type of surfactants Amount of Surfactant (g) Amount of Enzyme added (g) 1 Sodium Acetate Sodium Thiosulphate10.1 5Sodium Thiosulphate Pentahydrate Sodium Hydrogen Carbonate Sodium Lauryl Sulphate Centrifugation 3.1 The solutions were concentrated by centrifugation to separate and reduce the overall protein content. In this process, all the samples were centrifuged at about 4000rpm for 30 min. 2.2 After 24 hours, the latex solutions are added with 0.5 more gram of surfactant. -> stirred.->mixed well. Note : This surfactant was act as a salt for washing and dispersing 3.2 The latex ready for centrifugation 3.3 After 30 min, the solutions were separated into 3 layers: cream latex on the top, latex mix with water in the middle and the last layer was water itself. 3.4 The cream latex was taken out and dried in the fume box for 24 h. 4. Kjeldahl Method 4.1 A simplified flowchart showing the steps for the preparation and analysis of samples for protein and nitrogen determination. Latex Samples Kjedahl sample preparation : Add 100 ml of distilled water with 2 tablets of kjeldhal catalyst and mix with 15ml of sulphuric acid in the digestion flask Digestion Distillation Titration 3.5 The dried latex after 24 h.