Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production By Xin Li

To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant. Purposes

To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant. To produce cheaper soydiesel, more soy protein, and less expensive ethanol from the soybean plants. Purposes

Modeling Soybean Arabidopsis (Arabidopsis thaliana) Soybean (Glycine max)

Modeling Soybean Arabidopsis (Arabidopsis thaliana) Soybean (Glycine max)

Modeling Soybean Arabidopsis (Arabidopsis thaliana) Soybean (Glycine max)

Modeling Soybean Both are oilseed plants Arabidopsis has a mapped genome Insertion-induced Arabidopsis mutants are av ailable commercially

Modeling Soybean Both are oilseed plants Arabidopsis has a mapped genome Insertion-induced Arabidopsis mutants are av ailable commercially

Modeling Soybean Both are oilseed plants Arabidopsis has a mapped genome Insertion-induced Arabidopsis mutants are av ailable commercially Insertion-induced Arabidopsis mutants are available commercially

Insertion-Induced Mutation T-DNA Gene

Insertion-Induced Mutation

Wild-type

Hypersensitive mutant

Insensitive mutant

Methods Grew 300 seedlings of each of 58 different mutants and wild-type in:

Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose

Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose 6% sucrose

Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose 6% sucrose Measured root length of seedlings grown in glucose (Gibson, 2005)

Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose 6% sucrose Measured root length of seedlings grown in glucose (Gibson, 2005) Use spectrophotometry to determine anthocyanin levels of seedlings grown in sucrose (Nacry,1998)

Results

Neff and Chory (1998)

Results

Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.

Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose. The mutant’s genotype is SALK_

Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose. The mutant’s genotype is SALK_ The disabled gene in mutant SALK_ is at1g06230.

Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose. The mutant’s genotype is SALK_ The disabled gene in mutant SALK_ is at1g The gene contains a bromodomain protein

Future Work Grow SALK_ in 1% glucose and 1% sucrose

Future Work Grow SALK_ in 1% glucose and 1% sucrose Grow SALK_ in 1% sorbitol and 6% sorbitol

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Acknowledgement Dr. Sue Gibson Dr. Chunyao Li Ms. Lois Fruen Ms. Chelen Johnson Dr. Jacob Miller Ms. Virginia Amundson Breck Advanced Team Research

Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production By Xin Li