VDS Manitoba Diagnostic Laboratory Disease Report CAVP June 5, 2011 Guelph,Ontario

VDS MAFRI 2011 Egg Drop Syndrome? Rabbit hemorrhagic disease – household with 3 pet rabbits. One died others unaffected. Strain susceptibility Goat Abortion Herpesvirus – new introductions, existing ewes high rate of abortion. Typical herpes lesions in liver Herpes virus pneumonia zebra ILT ongoing in small farm flocks – provincially reportable. Flock destruction and compensation Turkey influenza –Low pathogenic avian influenza A H5N2 infection in a turkey breeder flock (~3000 birds) in late November Oropharyngeal swabs were submitted initially along with lung tissues with a history of some drop in egg production –Submitted on a Friday when staff sent home due to air quality. Not processed until Monday –What if high path AI?????

VDS MAFRI 2011 Recent increase in PRRS outbreaks –PRRS virus, mostly North American field strains, has been detected 9 separate cases in May TGE outbreak in sows in May Outbreak started in a sow barn and spread to a few nurseries. TGEV was detected by specific PCRs in VDS and Biovet. Outbreak was immediately contained. TGE outbreak in a finisher barn in February Outbreak was immediately contained panH1N swine influenza cases. Several cases in 2010 and only 3 cases in 2011 so far. H1N2 swine influenza virus has been detected in two cases recently H1N2 - being seen with increasing frequency – developing multiplex PCR to identify strains. Will do Matrix first and if required multiplex for typing. Porcine Myelopathy problem – holey spinal cord!

VDS MAFRI 2011 Bovine Anaplasmosis, - also Increasing recognition A. Phagocytophila in dogs. 6 herds with Mycoplasma suis and one PCR positive Mycoplasma ovis sheep flock – –emerging hemoparasites due to climate instability??? Increased case of liver flukes – all ruminants – study with CVO Liver fluke in bovine fetal liver – focal hepatic necrosis with fluke puke and fluke eggs. Injectable Se overdose in neonatal small ruminants – focal myocardial fibrosis and mineralization –High liver Se levels. S. zooepidemicus pneumonia outbreak in barn cats Septicemic P. multocida Type B in sheep Iridovirus-like Agent Sturgeon ( confirmed on EM in 2010) Lawsonia enteritis in horse

Egg Drop Syndrome? 6 flocks affected 20 – 30% production drop –Decreased egg #s, no shell, soft shell No change food or water intake Duration < 14 days 8 days Any stage of production One case with 10% decrease production –Associated with low dietary salt Ongoing disease investigation with CVO office –All test negative for infectious agents including duck adenovirus

Equine Proliferative Enteritis EPE

Rabbit Hemorrhagic Disease Household with 3 pet rabbits Most recent introduced 1 year ago Rescue rabbit, source unknown Died others unaffected Strain susceptibility Diagnostic pathologists will always be needed

Rabbit Hemorrhagic Disease

Liver flukes - bovine fetus

“Sick Building Syndrome” Aug with intermittent sewer gas odour. –Program issue (?) –Abandoned sewer pipes not capped Nov 16 – significant episode of respiratory discomfort by all Clin Path staff IAQ testing, building ventilation assessment, Occupational health physician June 3 – remedial work imminent One affected laboratory – impact on ACHSN