PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

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Presentation transcript:

PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

ASEPTIC PROCECURE FOR MICROBE REMOVAL http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related

ASEPTIC PROCECURE FOR MICROBE REMOVAL FOR PREPARATION OF A BACTERIAL SMEAR

http://www.youtube.com/watch?v=HxjN2mBLGCE&feature=related http://www.youtube.com/watch?v=n-HHI4E1LhI&feature=related

SPECIMEN PREPARATION FOR LIGHT MICROSCOPY PREPARATION OF A BACTERIAL SMEAR

STAINING SPECIAL STAINING TECHNIQUES Negative Staining for Capsules Capsules Determinants for virulence in some genera of bacteria Capsule Staining Preparation of a bacterial smear along with negative staining w/ Nigrosin or India Ink Counterstain w/ Crystal Violet

STAINING SPECIAL STAINING TECHNIQUES Negative Staining for Capsules Preparing a bacterial smear for Capsule Staining

STAINING SPECIAL STAINING TECHNIQUES Negative Staining for Capsules Capsule Stain Results

STAINING SPECIAL STAINING TECHNIQUES Endospore Stain Genera of bacteria that produce endospores Bacillus & Clostridium Other genera: Desulfotomaculum, Sporosarcina, Sporolactobacillus, Oscillospira, Thermoactinomyces Schaeffer-Fulton Method for Endospore Staining Preparation of a Bacterial Smear-Air dry Application of Primary Dye (Malachite Green) w/ concurrent application of heat Allow slide to cool Rinse w/ water Application of Counterstain (Safranin)

http://www.tgw1916.net/video_pages/Spore.html http://www.answers.com/topic/endospore

SPECIAL STAINING TECHNIQUES Endospore Stain Results http://www.tgw1916.net/movies.html SPECIAL STAINING TECHNIQUES Endospore Stain Results Flagellar Stain Uses mordant and carbolfuchsin stain to increase flagellar diameter until resolved by light microscope http://www.answers.com/topic/endospore

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