A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes

Gel is 22.5 x 22.5 cm in size Comb produces 28 wells

Uncut:DNA18.75 μl 10 x Buffer15 μl Water μl 6 x Loading Buffer30 μl 30 μl/well – approximately 1.25 μg DNA/well Single cut:DNA37.5 μl 10 x Buffer22.5 μl BamHI15 μl Water150 μl 6 x Loading Buffer45 μl 30 μl/well – approximately 1.67 μg DNA/well Bouble cut:DNA37.5 μl 10 x Buffer22.5 μl BamHI11.25 μl EcoRI11.25 μ l Water142.5 μl 6 x Loading Buffer45 μl 30 μl/well – approximately 1.67 μg DNA/well Each sample of DNA was incubated for 1 hour at 37 °C. Each sample and the ladder was then loaded into each well as outlined in the following slides. pBabe/cpp bp BamHI EcoRI 900 bp 5100 bp pBabe plasmid donated by Dr. Tang

Lane 1 Lane 10 Lane 20 Lane 28

2 3 1 Buffer Stain

27 17 Ladder Rows: 1

The SBI4U class of Hill Park Secondary School wish to thank Dr. D. Tang (McMaster University/St. Joseph’s Hospital) and his lab technician for donating the DNA, Restriction Enzymes and Ladder and for calculating the amounts of material required to complete this procedure successfully.