RENAISSANCE NUTRITION, INC. Presented By

Overview 1) What is Dairyman’s Edge ® PRO? 2) How it works 3) Proof it works 4) Economic benefits 5) Summation

What Is ? Nutritional tool designed to:  Maintain & improve rumen function  Improve overall gastrointestinal efficiency Successor to leading ProNutrient, Dairyman’s Edge ® All ingredients researched & selected for mode of action

What You Get With Live Yeast Yeast Culture Live Bacteria Active Enzymes Salt of Glutamic Acid 40.0 x 10 9 CFU/oz. 2.5 x’s DME Upgraded Yes + Additional Yes

How it works: Live Yeast Scavenges Oxygen ↓Oxidative stress ↑ Level of cellulolytic bacteria ↑ Total viable bacteria ↑ Microbial protein production ↑ Rate of fiber digestion ↑ VFA production ↓ Lag time of fiber digestion ↓ Lactate production Controls ruminal pH

How it works: Yeast Culture Nucleic acids Iso acids Vitamins Cofactors Enzymes Yeast Culture ↑ Total Rumen Bacteria

How it works: Bacteria Species supplied 1)Enterococcus faecium 2)Lactobacillus acidophilus 3)Lactobacillus plantarum 4)Lactobacillus brevis 5)Bacillus subtilus Modes of Action 1)Inhibit bad bacteria 2)Supply enzymes/ vitamins 3)Colonize small intestine 4)↑Rumen bacteria levels 5)↓Negative effects of stress

How it works: Enzymes Amylase, Cellulase, Betaglucanase, Hemicellulase, Xylanase ↑Fiber & starch digestion ↑Rate of carb assimilation ↑Microbial protein production ↑Available nutrients ↓Grain passage into manure ↓Viscosity of rumen fluid Breakdown feedstuffs into simpler substrates

How it works: Salt of Glutamic Acid Enhances feed palatability Transaminated into amino acids Bacteria absorb amino acids ↑ Microbial protein production

Add It All Together And You Get: +

Proof it works: Production 11 Commercial research trials Mid-late & early lactation groups Switchback & side x side designs Multiple states & seasons Control rations varied by farm All test rations included Dairyman’s Edge ® PRO Effect of Dairyman’s Edge ® PRO on Production Performance

Statistical Analysis 11 Commercial Trials 2 ADFI Metabolic Trials Protocol Review & Statistical Analysis Dr. Essi Evans of Essie Evans Technical Advisory Service

Production Performance Results Mid-Late lac. Averages 150 Day Milk lbs. % Protein % Fat Early lac. Averages Milk lbs. % Protein % Fat +0.15

Proof it Works: Rumen Digestion Atlantic Dairy and Forage Institute (ADFI) Establish effect of Dairyman’s Edge ® PRO on Feed digestibility 4 rumen fistulated cows Test cows fed PRO Various feedstuff samples Sample incubation periods of 6, 12, 24 hrs. How Where Why

Rumen Digestion Results Effects Of Dairyman’s Edge ® PRO On Feedstuff Digestibility Feedstuff% NDF% ADF Change In NDF Digestion Change In ADF Digestion Solubility Corn Silage49.0%29.0% ↑ 1.00% ↑ 4.0% ↑ 0.40% Alfalfa Silage47.0%36.0%Unchanged ↑ 7.3% ↑ 2.08% Timothy Hay69.0%36.0% ↑ 5.00% ↑ 2.00% ↑ 4.23% Dry Corn Grain13.0%3.00% ↑ 5.50% Unchanged ↑ 15.4% Soybean Meal9.50%7.50% ↑ 3.00% ↑ 1.80% ↑ 14.5% Digestibility improvements = ↑ Glucose for rumen microbes

Proof it works: Whole Tract Digestibility Research by ADFI to determine effect of PRO on:  Urinary CP & Metabolizable protein 8 cow side x side design 4 fed Dairyman’s Edge ® PRO 2 week period Last 4 days in metabolism stall

Whole Tract Digestibility Results Group Crude Protein (N) Loss in Urine Metabolizable Protein Available for Milk etc. Control Ration Dietary Crude Protein 41.2%29.7% Pro Treatment Ration Dietary Crude Protein 32.2%37.0% Difference-9.0%+7.3% Dairyman’s Edge ® PRO= Less protein wasted in urine; More microbial protein production

Economic Benefits $1.23/ lb. $2,460/ ton. $0.077/ head per day 50 lbs./bag 40 Bags/ Skid Milk Price/ cwt Increased Production IncomeCost PRO Benefit $ lbs.$0.68$ $0.51 $ lbs.$0.76$ $0.68

Summation What you can expect with Solid Ingredients Live Yeast/ Yeast Culture Bacteria Enzymes Salt of glutamic Acid Solid Results ↑Production Performance ↑ Nutrient Utilization ↑ Return on investment ↓ Nutrient Wasting