Sudden Death and Sickle Cell Trait: How Knowing Your Genes Can Save Your Life Dale Lloyd II, No. 39. (The Rice Football Webletter) 2006 After running 16 consecutive sprints of 100 yards each, he collapsed. He died the next day from acute exertional rhabdomyolysis (ER) associated with a genetic condition called sickle cell trait. Learning Set 4 : Lesson 1 : Slide 1

Normal Blood Cells Sickle Cell Trait Blood Cells Sickle Cell Disease Blood Cells Learning Set 4 : Lesson 1 : Slide 2 Blood Cells

View video on sickle cell trait Interview with sickle cell patient: pain Learning Set 4 : Lesson 1 : Slide 3 View Video on sickle cell anemia Sickle Cell Disease

Red Blood Cell Oxygen molecule Hemoglobin subunit oo Learning Set 4 : Lesson 1 : Slide 4 Hemoglobin

Red Blood Cell oxygen molecule (O 2 ) Hemoglobin subunit Learning Set 4 : Lesson 1 : Slide 5 Hemoglobin (transport protein)

6 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

7 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

8 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

9 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

10 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

11 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

12 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

13 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

14 Hemoglobin Learning Set 4 : Lesson 1 : Slide 6

Learning Set 4 : Lesson 2 : Slide 7 Electrophoresis of Sickling and Non Sickling Hemoglobin

Learning Set 4 : Lesson 2 : Slide 8 Electrophoresis of Sickling and Non Sickling Hemoglobin

Chromosome 11 Learning Set 4 : Lesson 2 : Slide 9 Chromosomal Location of Hemoglobin

Learning Set 4 : Lesson 2 : Slide 10 Mutation found in Hemoglobin

Learning Set 4 : Lesson 2 : Slide 11 Mutation found in Hemoglobin

Charge –Positive and negative charged amino acids attract each other –Amino acids with the same kind of charge repel each other Hydrophobicity –Hydrophilic amino acids attract water –Hydrophobic amino acids repel water and hydrophilic amino acids –Hydrophobic amino acids attract other hydrophobic amino acids. Cysteines –Cysteines are a type of amino acid. When two Cysteines are next to each other they attract and form a strong bond. These strong bonds contribute to protein shape formation. Red = positive charge (+) K, R, H Yellow = negative charge (-) D, E Green = hydrophobic A, V, L, I, P, M, F, W Blue = hydrophilic G, S, T, N, Q, Y White = cystine C Learning Set 4 : Lesson 2 : Slide 12 “Rules” that amino acids follow

Learning Set 4 : Lesson 2 : Slide 13 Sickle Cell Hemoglobins Stick Together

Learning Set 4 : Lesson 2 : Slide 14 Electrophoresis of Sickling and Non Sickling Hemoglobin

L T P E E K S L T P V E K S DNA order of amino acids protein shape cell function Learning Set 4 : Lesson 2 : Slide 14 Effects of Sickle Cell Mutation

Learning Set 4 : Lesson 3 : Slide 16 Passing on Mutations

Learning Set 4 : Lesson 4 : Slide 17 Frequency of Sickle Cell Causing Mutation vs. Frequency of Malaria Causing Microbe

Analyzing RNA Using Gel Electrophoresis Learning Set 4 : Lesson 5 : Slide 18

Who has lactose intolerance in Jason’s family? Learning Set 4 : Lesson 5 : Slide 19

DNA Sequence Analysis of Lactase Protein Gene Learning Set 4 : Lesson 5 : Slide 20

Gel Electrophoresis: Lactase Protein Samples from Jason’s Family Learning Set 4 : Lesson 5 : Slide 21

RNA Analysis of Lactase Protein Gene Learning Set 4 : Lesson 5 : Slide 22

Learning Set 4 : Lesson 5 : Slide 23 DNA Sequence of Area Near the Lactase Gene