ACIS ® III Image Analysis Robert Carlson Manager, Imaging Systems *Accurate *Efficient *Consistent *Easy to use

DAKO: 40 Years Experience in AP Marketplace From Antibodies to System Solutions 1966 Antibody Production 1995 TechMate ™ 2000 Dako Autostainer ™ 2002 Artisan ™ 2006 ACIS ® III 2007 The Dako Link Solution

Addressing Your Challenges Cost and Productivity Turn Around Time Quality  Utilize labor time more efficiently  Simplify ordering processes  Streamline workflow  Eliminate redundant tasks  Process simultaneously  Reduce repeats  Improve consistency  Implement cutting-edge technologies

ACIS ® III is Accurate  Proprietary, color-based image analysis software combined with Whole Slide Scanning automated microscopy  FDA Cleared Applications  HercepTest TM for HER2  ER  PR  Additional applications  Generic applications (Membrane, Nuclear, Microvessel Density, Ploidy, Rare Event)  Tissue Micro Array (TMA)  Flexible solutions to meet unique research needs

ACIS ® III is Efficient  Technician Operation  Automated calibration (10 min., once every 24 hours)  Accessioning  Dako LINK software Integration  ACIS III accessioning screen  Automated processing of slides  High capacity (100 slides)  Continuous access 24 hours a day  Immediate access to digital slides for scoring  Analysis of digital slides by pathologist  Need a quick consult or second opinion…use Dako EyeShare TM  Report generation by pathologist or technician

ACIS ® III is Consistent  Gold Standard Calibration  Daily, one-touch calibration ensures proper focus, color balance and light intensity  Calibration does not require additional pathologist time  ACIS III improves inter-observer reproducibility with consistent stain intensity scoring Proper calibration helps ensure laboratory results are consistent

 4-tab Dako software format  Accessioning  Microscope  Analysis  Report  Easy to use  Reduces training time  Reduces hands-on time ACIS ® III is Easy to use Software

Dako EyeShare™  Remote Slide Viewing Software via the internet  Live Consultation  Second opinions  Teaching  Standardization

ACIS: Proven Performance : Sample Article Listing Most peer reviewed imaging system on market ACIS ® Selected Article Bibliography Automated System Improves Accuracy of HER-2 Scoring. ONI, April Ayala G, Wang D, Wulf G., Frolov A, Li R, Sowadski J, Wheeler T, Lu K, Bao L. The Prolyl Isomerase Pin1 is a Novel Prognostic Marker in Human Prostate Cancer. Cancer Research 2003; Vol. 63, Bauer KD, Torre Bueno, J, Diel IJ, Hawes D, Decker WJ, Priddy C, Bossy B, Ludmann S, Yamamoto K, Masih AS, Espinoza FP, and Harrington DS. Reliable and Sensitive Analysis of Occult Bone Marrow Metastases Using Automated Cellular Imaging. In Autologous Blood and Marrow Transplantation X: Proceedings of the 10th International Symposium (K.A. Dicke and A. Keating, eds) Carden Jennings Publishing Co., Charlottesville, VA, Becker S, Becker-Pergola G, Fehm G, Emig R, Wallwiener D, Solomayer E-F. Image Analysis Systems for the Detection of Disseminated Breast Cancer Cells on Bone-Marrow Cytospins. Journal of Clinical Laboratory Analysis 19:115–119, Birner P, Ritzi M, Musahl C, Knippers R, Gerdes J, Voigtlander T, Budka H, Hainfellner J. Immunohistochemical Detection of Cell Growth Fraction in Formalin-Fixed and Paraffin-Embedded Murine Tissue. Am J Pathol 2001; Vol. 158,

Summary of Key Benefits of Dako Solution True Positive ID Support ConnectivityOptimal Staining Performance Easy-to-Use Software