MONITORING IN UNIVERSITY LABS PI: M. Ellenbecker (Toxics Use Reduction Institute) Post-doc: S. Tsai Funding from NSF EEC-0425826 for the Nanoscale Science.

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MONITORING IN UNIVERSITY LABS PI: M. Ellenbecker (Toxics Use Reduction Institute) Post-doc: S. Tsai Funding from NSF EEC for the Nanoscale Science and Engineering Center for High-rate Nanomanufacturing is gratefully acknowledged. J. Isaacs, A. Busnaina J. Mead, C. Barry TOXICITY SCREENING PIs: D. Bello (Work Environment), E. Rogers (Clinical Laboratory Sciences) Graduate RA: S. Hsieh Tested 3 hood types Leaking of NPs were found, caused by complex airflow pattern Exposures vary by hoods, operating conditions, worker’s motions and other vaiables. Standard hood test allowed 0.1ppm of tracer gas release, this can have millions of NPs being released Solutions: need to improve practices and evaluate other new hood designs Exposure Monitoring of Airborne Nanoparticles in University Laboratories Abstract : The Center for High-rate Nanomanufacturing (CHN) is committed to working safely with nanoparticles (NPs). As part of this commitment, we have been evaluating potential nanoparticle exposures in CHN labs and developing best practices for handling nanoparticles. We monitored airborne nanoparticle exposure at various workplaces, studied 7 different processes and 7 types of nanomaterials. In addition, since m we evaluate the performance of 3 types of fume hoods to against nanoparticle exposure Abstract : The Center for High-rate Nanomanufacturing (CHN) is committed to working safely with nanoparticles (NPs). As part of this commitment, we have been evaluating potential nanoparticle exposures in CHN labs and developing best practices for handling nanoparticles. We monitored airborne nanoparticle exposure at various workplaces, studied 7 different processes and 7 types of nanomaterials. In addition, since manual handling of nanoparticles is a fundamental task of most nanomaterial research; such handling may expose workers to ultrafine or nanoparticles, we evaluate the performance of 3 types of fume hoods to against nanoparticle exposure. Equipment : Fast Mobility Particle Sizer Model 3091 (FMPS) by TSI is used to measure particles size range from 5.6 to 560 nanometers in 32 size channels with 1 sec sampling time. Collect nanoparticles on Cu/C tapes, TEM grids and polycarbonate filters at breathing zone using filtration method. Workplace monitoring Fume hood experiments Studied 7 NPs, 7 processes Measured NPs release on most processes Evaluate ventilation - fume hood Engineering controls at source reduced nanoparticle release Administrative controls removed background nanoparticles Need good work practices to reduce exposure Transferring Pouring Publications and Presentations Tsai, S., Ashter, A., Ada, E., Mead, J., Barry, C., Ellenbecker, M., “Airborne Nanoparticle Release Associated with the Compounding of Nanocomposites using Nanoalumina as Fillers,” Journal of Aerosol and Air Quality Research, Vol. 8, No. 2, , June Tsai, S., Ada, E., Ashter, A., Mead, J., Barry, C., Ellenbecker, M., “Control of Airborne Nanoparticles Release during Compounding of Polymer Nanocomposites,” in press, NANO 3(4):1-9 (2008).. Ashter, A., Tsai, S., Ellenbecker, M., Mead, J., Barry, C., “Effects of Nanoparticle Feed Location during Nanocomposite Compounding,” accepted to Polymer Engineering and Science Journal, Ellenbecker, M., Tsai, C., Issacs, J., “Best Practices for Working Safely with Nanoparticles in University Research Laboratories,” submitted to special issue of the International Symposium on Nanotechnology in Environmental Protection and Pollution occupational hygiene paper in preparation 5 presentations in 5 conferences Publications and Presentations Tsai. S., Ada, E., Isaacs, J., Ellenbecker, M., ”Airborne nanoparticle Exposures Associated with the Manual Handling of Nanoalumina and Nanosilver in Fume Hoods,” Journal of Nanoparticle Research, July presentations in 4 conferences Initiated cooperation with hood researchers in Taiwan Results of handling 100 g nanoalumina in the conventional hood Results of isolating feeding port of nanocomposite compounding process Nanoalumina particles collected at breathing zone Transferring Pouring Breathing zone concentration at conventional hood (background subtracted) Nanoalumina particles collected at breathing zone MONITORING Characterization Integrated Approaches Biological Significance Metrics Biomarkers Good Work Practices Engineering Controls PPE, etc. Nano silver in serum mix by EM and confirmation by EDS “Oxidant activity has been shown to be a property of particulate matter and its components that reflect crucial biological mechanisms.” Borm et al. Occup Environ Med 2007; 64: TSI FMPS 3091 Naneum WRASS 05 integrated sampling (1 nm-35 um in 12 stages) for microscopy characterization (EM) and chemical analysis Thermal precipitator for EM & EDS PUBLICATIONS A high throughput analytical approach to screen for oxidative damage potential of nanomaterials in a biologically relevant system: human blood serum. Hsieh S-F, Bello D, Rao N, Rogers E (UML). submitted Exposure to nanoscale particles during CVD growth and subsequent handling of vertically-aligned CNT films Bello D, Hart AJ, Ahn K, Hallock M, Yamamoto N; Garcia E, Ellenbecker MJ, Wardle BL (UML & MIT), submitted Exposure to nanoscale particles during fabrication and machining of modern hybrid and nanocomposites Bello D, Hallock M, Ahn K, Yamamoto N; Garcia E, Ellenbecker MJ, Hart AJ, Wardle BL (UML & MIT), submitted Exposure to nanoscale silver particles associated with antimicrobial coatings Bello D, Ahn K, Ada E, Ellenbecker M (UML); submitted Research Thrust 4 Social & EHS Implications Northeastern University, University of Massachusetts Lowell, University of New Hampshire