Prepared by Farid Khalaf

 Protect the worker.  Protect the sample being analyzed.  Protect the environment

 Is a chamber generally constructed of steel. It has :  Front glass window of adjustable height,  Ventilation system with an electrical motor generating negative pressure inside the cabinet.  High-Efficiency Particulate Air (HEPA) filter (Removes 99.99% of 0.3 µm particles).

 Class I Biological Safety Cabinet  Class II Biological Safety Cabinet  Class III Biological Safety Cabinet

 The Class I biosafety cabinet provides protection to the operator and the environment but not the sample in the cabinet  It has similar air flow as a chemical fume hood, but has a HEPA filter in the exhaust.  This older class of biosafety cabinet is rarely seen in biomedical and microbiological laboratories.

 The Class II BSC is an open-front, ventilated cabinet.  Protection offered: To the operator, the sample and environment.  Suitable for working with agents with biosafety level 1, 2 or 3.  Filtration system: two HEPA filters,  They recycle approximately 70 % of the air volume and renew 30 % of it.  While HEPA filters are effective for trapping particulates and infectious agents, these filters will not capture volatile chemicals or gases.

 The Class III cabinet is a totally enclosed ventilated cabinet.  Operations within the Class III cabinet are conducted through attached rubber gloves.  Supply air is drawn into the cabinet through HEPA filters. The cabinet exhaust air is filtered by two HEPA filters, installed in series, before discharge outside of the facility.  Protection provided: to the operator, the product and the environment.  Suitable for working with agents classified biosafety level 4.

 Offer only personnel protection  Always exhaust air to the outside  Do not offer protection to the product or the environment, as there is no filtration of intake and exhaust air (Sometimes air cleaning treatment is added to the exhaust.)  Used for work with chemical hazards

1.A laboratory area protected from air currents from windows or air-conditioning systems. 2.An electrical connection equipped with the respective control and safety elements; the electrical outlet with a ground pole. 3.The floor on which it is located must be flat and leveled. 4.The free space around the cabinet and the height of the room recommended by the manufacturer must be respected. 5.Gas connections must be in the immediate vicinity of the cabinet in order to facilitate the connection to these service valves. 6.The cabinet must be certified annually.