Chromatography 研習資料 Separation of Pigments In a solvent according To their densities

Separate plant pigments

Also for Forensics Find type of ink (different color separations) Find impurities in products

Taper Strip Cut to a point with scissors

Mark a starting line Use pencil (not ink)

Chemical Application Add small drops, let dry, add again until dark spot KEEP ABOVE PENCIL LINE

Use coin (mechanical) – geranium leaf Use serrated coin Do a few times to make a dark line

Be sure liquid is below line Attach paper to paper clip Attach paper clip to stopper

Chromatography Animation Paper Chromatography Videos

Chromatography Over Time Time Lapse Video (Be patient and give it time to move)

Mark bands of colors Lighter pigments will move farther up the paper Heavier pigments will remain near the start

Pencil line will mark start point

Separation of Ink

Calculating R f Compares distance the solvent traveled to the distance the test sample traveled. Rf = distance of test sample distance solvent

Measure tops of colors From starting point at bottom to top of each color Distance of solvent

Pigments in Plants carotene-yellow orange chlorophyll a-bright green Xanthophylls-yellow chlorophyll b-khaki green

More colors phycocyanin = bluish phycoerythrin = reddish

Carrier Solution Stored in da hood! Return the waste to the waste container in da hood. Boy-e-e-e-e-e-e-e-e-e-e!

Sample Results

Click Here for Examples Results of Chromatograph

Accessory Pigments fucoxanthin = brown Phycobilin = reds phycocyanin = bluish

Conclusion Question #2