In vitro evaluation of biomaterials for bone tissue engineering Biomaterials Dept. Materials Science and Technology University of Crete, P.O. Box 2208.

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Presentation transcript:

In vitro evaluation of biomaterials for bone tissue engineering Biomaterials Dept. Materials Science and Technology University of Crete, P.O. Box 2208 GR Heraklio, Crete, Greece Maria Chatzinikolaidou

Research activities In vitro biocompatibility study of biomaterials, including cell adhesion, viability, proliferation and morphology of mammalian cell lines and stem cells Investigation of the osteoinductive potential of implantable biomaterials  bone tissue repair Development of immobilization techniques for proteins on biomaterials surfaces Adsorption and desorption studies of proteins on biomaterials surfaces

Cell adhesion shown by SEM Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest Biomaterial: organic-inorganic composite material, structured by two- photon polymerization After 3 days In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki After 4 hours

Cell adhesion and proliferation shown by SEM Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest Biomaterial: organic-inorganic composite material, structured by two- photon polymerization After 6 days In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

Cell adhesion shown by SEM Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest Biomaterial: organic-inorganic composite material – meshes structured by two-photon polymerization After 4 hours In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

Cell formations during adhesion Extracellular factors and integrin-derived signaling trigger activate actin polymerization at the leading-edge membrane (as an early event) and the formation of focal adhesions (as a later event) Early event Biomaterials: The Intersection of Biology and Materials ScienceJohnna S. Temenoff, Antonios G. Mikos Late event

Cell morphology of MC3T3-E1 Visualization of vinculin and actin cytoskeleton by means of confocal microscopy Overlay of double staining with ΤRΙTC-conjugated falloifin and FITC-conjugated anti-vinculin magnification 20x Cell source: mouse pre-osteoblasts MC3T3-E1 Biomaterial: organic-inorganic composite material

Cell morphology Visualization of vinculin and actin cytoskeleton by means of confocal microscopy Overlay of double staining with ΤRΙTC-conjugated falloifin and FITC-conjugated anti-vinculin magnification 40x Cell source: mouse pre-osteoblasts MC3T3-E1 Biomaterial: organic-inorganic composite material

Acknowledgements Collaborations Prof. Maria Vamvakaki, UoC Dr. Maria Farsari, Prof. Costas Fotakis, IESL-FORTH Prof. Eleni Papadaki, UoC

A reservoir for progenitor cells

Organic-inorganic composite material Methacryloxypropyl trimethoxysilane (MAPTMS), (2-dimethylamino)ethyl methacrylate (DMAEMA) and methacrylic acid (MAA) as the polymerizable monomers Zirconium n-propoxide (Zr(OPr) 4, and the trimethoxysilane groups of MAPTMS serve as the inorganic network forming moieties