(1) Assess benthic faunal colonization on banks (2) Assess trophic status of restored salt marshes Levin Lab Benthic Studies Goal: Develop metrics for.

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(1) Assess benthic faunal colonization on banks (2) Assess trophic status of restored salt marshes Levin Lab Benthic Studies Goal: Develop metrics for healthy salt marsh and subtidal habitat

Bank Macrofauna What are macrofauna? small worms, clams, crustaceans and other invertebrates Significance of macrofauna: major agents of sediment stability/instability primary food items for bottom feeding fish, shrimp Strategy: sample macrofauna at time intervals following bank placement Sampling and analysis methods field - sediment coring, vertical sectioning, preservation laboratory - sieving, sorting under dissecting microscope, counting, species identification (~ 1 da/sample) statistical measures of diversity and community structure What we learn: proxy for ecosystem successional state and health

Salt Marsh Trophic Function What is Trophic Function? A key ecosystem property, Food web and energy transfer Goal: Develop a method for assessing recovery of trophic function in restored Venice Lagoon salt marshes Rationale: Most measures of recovery examine structural properties rather than function Approach: Use stable isotopic analyses to examine food web organization (isotopic signatures differ for different plants/algae and are passed from food to consumer) Compare food webs in natural and restored salt marshes of different ages Methods: Sample primary producers and consumers, dry and powder Analyze isotopic signatures (  13 C,  15 N) on a mass spectrometer. Use mixing models to establish trophic structure

What we learn What forms the base of the food chain in undisturbed marshes? How many trophic levels are present in a healthy marsh? Are these similar in restored marshes? After how many years? Can we develop a functional measure of marsh recovery? Salicornia?Spartina? Limonium? Cyanobacteria? Phytoplankton?