Instructions To use this template: –for each slide write the correct answer on the orange bar first –choose which option (A,B,C or D) and make sure you write the letter on the orange bar in place of the question mark –Now drag the orange bar on top of the correct option so it sits exactly over the top - now when you click through the onscreen animation, the correct answer will appear to be illuminated –View the next slide to see how it should look

$1,000,000 Example of how it looks when complete, click on-screen show to view the animations B - correct answer A - answer C - answer D - answer B - correct answer

Who wants to be a Millionaire? Basic Sciences Translational Research Hosted by CUREiculum

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The Prizes 1 - $ $ $ $1, $2, $4, $8, $16, $32, $64, $125, $250, $500, $1,000,000

Lifelines “Phone a friend” Ask the audience

$100 Which word is greatly associated with CD4+ T cells? B - Reservoir A - Latency C - Memory D – All of the above

$200 Memory T cells can last for ____? B – Several decades A – Several days C – Single year D – Seven years B – Several decades

$500 Where does HIV persist during ART? B - Heart A - Stomach C – muscle tissue D - Brain

$1,000 What are histones? B – HDAC inhibitors A – Special HIV genes C – Cellular proteins D – Latently infected cells C – Cellular proteins

$2,000 According to studies, the size of the HIV reservoir is what overtime? B – Increases A – Remains stable C – Decreasees D – Disappears A – Stable

$4,000 What is associated with having a smaller reservoir? B – Higher CD4+ count A – Late initiation of ART therapy C – Lower CD4+ count D – Stagnant CD4+ count B – Higher CD4+ count

$8,000 Which of the following does Antiretroviral drugs not do? B – Eradicates HIV A – Suppress HIV to undetectable levels C – Restricts size of the HIV reservoir D – A and C B – Eradicates HIV

$16,000 How many proven strategies currently exist to eliminate the reservoir? B – 1 A – 0 C – 10 D – 4 B - 1

$32,000 Where can the most number of CD4+ T cells found? B – Thymus A – Lymph node C – Gut D – Spleen A – Lymph node

$64,000 What is another name for negative regulators? B – Immune receptors A – Latent regulators C – Autoimmune receptors D – Immune checkpoints

$125,000 CD4+ T cells expressing PD-1 have ______ levels than other CD4+ T cells : B – similar A – lower C – higher D – more defective C – higher

$250,000 Which is a concern when targeting immunological mechanisms of HIV latency? B – Immune depletion A – Proliferation of cells C – Autoimmunity D – Increased risk of infection C – Autoimmunity

$500,000 The total number of reservoir CD4+ T cells in suppressed individuals is? B – 10, ,000 A – 1,000-10,000 C – 100,000-1,000,000 D – 100,000-10,000,000

$1,000,000 What is the largest part of the HIV reservoir? B – Intact non-induced proviruses, inducible after additional rounds of stimulation A – HIV proviruses induced after one round of maximum T cell activation C – Intact non-induced proviruses D – HIV-1 DNA

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