Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research.

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Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research

H&E and SHG Protocol – Thaw & fix sample – Section sample to 200 microns on a vibratome – Image with SHG – Paraffin embed tissue wafer – Section and stain for H&E – Slide scan Green = SHG signal (Collagen Type I)

Normal Associated Tissue 11.6 mm H&E SHG

Invasive Ductal Carcinoma H&E SHG

Fibroadenoma H&ESHG

7.5 mm Mucinous Carcinoma H&E SHG

Intrinsic Fluorescence Green = SHG Red = Autofluorescence from FAD or Collagen type III

FibroAdenoma

Extrinsic Fluorescence Green = SHG (Collagen type I) Red = E-Cadherin stained cells & Autofluorescence from Collagen type III

Terminal Ductal Lobular Unit (Red) Surrounded by Collagen (Green)

Duct in Cross Section, Surrounded by IDC cells IDC Duct

IDC Cells Near a Vessel with ECM IDC Cells Vessel