Using modified collagen scaffolds to promote angiogenesis for tissue engineering applications Julie M. Etheridge Tissue Engineering and Biomaterials Laboratory Fischell Department of Bioengineering University of Maryland, College Park, MD A. James Clark School of Engineering RISE Leadership Academy

Leadership in Research Project Objective: To promote the formation of pre-vascular networks in engineered tissue constructs by immobilizing an angiogenic growth factor (platelet derived growth factor, PDGF-BB) on collagen scaffolds 1. Immobilize growth factor on 3-dimensional collagen hydrogel scaffolds 2. Examine effect of growth factor on vascularization of human umbilical vein endothelial cells (HUVECs) in monolayer Project Significance: Bridge the gap between tissue engineering and clinical/industry applications by developing functional tissue grafts for implantation

End of Semester Report Project Conclusions: Objective 1 Collagen hydrogels successfully modified with PDGF-BB Objective 2 Low concentration of PDGF-BB induces long term angiogenic marker expression High concentration of PDGF-BB inhibits rapid endothelial sprout formation PDGF-BB induced pre-vascular network Collagen hydrogels containing encapsulated HUVECs

Leadership in research Presented project findings as a representative of the University of Maryland at the 2014 ACC Meeting of the Minds undergraduate student research conference