Lecture 12: Spermatogenesis

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Presentation transcript:

Lecture 12: Spermatogenesis Animal Science 434 Lecture 12: Spermatogenesis

Spermiation

Temporal Relationships of Spermatogenesis

Spermatogenesis 57 days Stallion

Multiple Generations of Germ Cells

Bull Spermatogenesis

Stages Specific cellular associations within a small segment of a seminiferous tubule stages are not the same length in time

Bull Spermatogenesis - Stages

Stages Specific cellular associations within a small segment of a seminiferous tubule stages are not the same length in time

Bull Spermatogenesis - Stages

Cycle progression through sequence of all stages

Bull Spermatogenesis - Cycles

Cycle progression through sequence of all stages 4.7 cycles to form spermatozoa some species variation

Duration of Spermatogenesis Bull Ram Boar Stallion Man cycle (days) 13.5 10.4 8.3 12.2 16 Spermatogenesis 61 47 39 57 75

Coordination of Stages Form Waves some portion of seminiferous tubule is always releasing sperm

Compartmentalization of Spermatogenesis and the Blood Testis Barrier

Seminiferous Tubule and Interstitial Tissue

Interstitial Tissue Vascular System Provides Nutrients Oxygen Blood Vessel Vascular System Provides Nutrients Oxygen Growth Factors Hormones

Blood Vessel

Compartments Tight Junctions Myoid Cells Luminal Adluminal Basal

Cycles and Stages

Compartmentalization Basal mitosis spermatogonia (A, I, B) primary spermatocytes Adluminal meiosis secondary spermatocytes spermatids spermiogenesis Lumen spermiation

Blood Testis Barrier Sertoli cells and associated tight junctions first appears at puberty induced by FSH after puberty can be maintained by FSH and/or testosterone essential for meiosis !!!!!

Capacity for Sperm Production Species Testis Sperm/gm DSP Weight (gm) (X 106) (X 109) Dairy Bull 650 18 10 Beef Bull 500 18 8 Ram 550 26 14 Boar 750 30 25 Stallion 165 18 3 Man 40 4 .045 - .2

Duration of Spermatogenesis Bull Ram Boar Stallion Man cycle (days) 13.5 10.4 8.3 12.2 16 Spermatogenesis 61 47 39 57 75

Spermatogonial Renewal in the Primate Ap Spermatogonial Renewal in the Primate Ad Stem Cell Pool FSH B P

Capacity for Sperm Production Species Testis Sperm/gm DSP Weight (gm) (X 106) (X 109) Dairy Bull 650 18 10 Beef Bull 500 18 8 Ram 550 26 14 Boar 750 30 25 Stallion 165 18 3 Man 40 4 .045 - .2

Variation in Sperm Production Testis Size Efficiency of spermatogenesis mitotic division degeneration of germ cells Length of spermatogenesis

Apoptosis of Germ Cells Season Disease Trauma or heat Hormone levels Normal part of spermatogenesis

Over Population of Spermatogonia Tight Junction Sertoli Sertoli SG SG SG SG SG SG SG SG SG Basement Membrane Normal Apoptosis Inhibited PS SG Basement Membrane Sertoli AP SG Basement Membrane Sertoli

Animation of Spermatogenesis