©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D.

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©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D.

SUGAR-PHOSPHATE BACKBONE H P O HO O O CH 2 HOH P O O HO O O CH 2 H P O OH HO O O CH 2 NH 2 N N N N O O N NH N N N O NH 2 N B A S E S DNADNADNADNA O H P O HO O O CH 2 HO O H2NH2N N HN N N H H P OH O O CH 2 O O N O H2NH2N N H H2OH2O HOH P O O O CH 2 CH 3 O O HN N H2OH2O

©2000 Timothy G. Standish The Watson - Crick Model Of DNA Major groove Minor groove A T T A G C C G G C T A A T G C T A A T C G

©2000 Timothy G. Standish Submarine Gel Electrophoresis Gel Box Negative electrode Positive electrode Sample to be loaded WellGel

©2000 Timothy G. Standish Submarine Gel Electrophoresis

©2000 Timothy G. Standish 100 V Submarine Gel Electrophoresis +- DNA Migration

©2000 Timothy G. Standish Gel Electrophoresis Wells

©2000 Timothy G. Standish Gel Electrophoresis Wells

©2000 Timothy G. Standish Gel Electrophoresis + - Direction of DNA Travel Wells Small Large

©2000 Timothy G. Standish Electrophoresis of Dyes

©2000 Timothy G. Standish