Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A Selection of recombinant E. coli cells Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite Digestion of pBR322  BamH1 with BamH1 and EcoRI restrictionenzymes Quality control by electrophoreses

Digestion of pBR322 with BamH1

Elongation of BamH1 restrictionsites, making of blunt ends

Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends

Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322

Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A

Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A Selection of recombinant E. coli cells

Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A Selection of recombinant E. coli cells Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite

Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A Selection of recombinant E. coli cells Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite Digestion of pBR322  BamH1 with BamH1 and EcoRI restrictionenzymes

Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A Selection of recombinant E. coli cells Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite Digestion of pBR322  BamH1 with BamH1 and EcoRI restrictionenzymes Quality control by electrophoreses

Preparation of competent E. coli cells Transformation of the ligated construct into E. coli Purification of the digested fragment Purification of the pBR322  BamH1 plasmid Digestion of pBR322 with BamH1 Elongation of BamH1 restrictionsites, making of blunt ends Ligation of the blunt end in pBR322A Selection of recombinant E. coli cells Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite Digestion of pBR322  BamH1 with BamH1 and EcoRI restrictionenzymes Quality control by electrophoreses