1% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 4,5 cm Running voltage: 100.

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1% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 4,5 cm Running voltage: 100 Volt Solidification time: 14 minutes Composition: 250 mikroL buffer TAE + 2,5 gram Agarose + 20 mikroL Ethidium Bromide 1 Kb 100 Kb Second Band Scale in Cm

2% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 3.4 cm Total Running time: 62 minutes Total distance measured: 4.5 cm Running voltage: 100 Volt Solidification time: 21 minutes Composition: 500 mL buffer TAE + 10 gram Agarose + 40 mikroL Ethidium Bromide 1 Kb 100 Kb Second Band Scale in Cm

3% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 2.9 cm Total Running time: 68 minutes Total distance measured: 4.5 cm Running voltage: 100 Volt Solidification time: 17 minutes Composition: 250 mikroL buffer TAE gram Agarose + 40 mikroL Ethidium Bromide 1 Kb 100 Kb Second Band Scale in Cm

Overall comparison Cross sections of 3 different concentration of DNA Agarose Gel are illuminated under UV light 1%2%3%

Overall Comparison 1% 2% 3% 1 kb 100 bp Increasing the concentration of Agarose gel decreases the electrpphoretic mobility of DNA Larger Fragments are better resolved in the low agarose concentration while higher concentration allow resolution of smaller DNAs

Comparison 5 mikroL of 1 Kb DNA Ladder in 1%,2% and 3% Agarose Gel Electrophoresis 1% 2%3% Wells level 3,0 kb 2,0 kb

Comparison 5 mikroL of 100 bp DNA Ladder in 1%,2% and 3% Agarose Gel Electrophoresis 1%2%3% Wells level 1000 bp 500 bp