Series II 2100 bioanalyzer kits Superior RNA 6000 assays and reagents

2 Improvements of the RNA assays Improved RNA ladder which is now included in both RNA kits Consistently higher sensitivity for the RNA Pico assay Increased assay stability for samples with a high salt content for both RNA assays New quantitation calibration for both RNA assays Larger marker volumes included Shelf life initially increased to at least 4 months. Extension is being tested Reagents are conveniently packaged according to storage temperature

3 New RNA ladder RNA ladder now included in the kits Ladder can also be ordered separately Provides more intense and stable bands More reliable integration and quantitation with the 2100 expert software Series II RNA 6000 Nano assay Old RNA 6000 Nano assay Ladder

4 Increased sensitivity 50 pg/µL total RNA in water was analyzed using the Series II RNA 6000 Pico assay Normal view Enhanced RNA view with the expert SW Sensitivity

5 Salt tolerabilty Series II assay tolerates high amounts of salt RNA 6000 Pico - Series II water 50 mM Tris 10 mM MgCl 2 50 mM NaCl RNA 6000 Pico - old 1 ng/µL total RNA was run in different salt-containing media water 50 mM Tris 10 mM MgCl 2 50 mM NaCl Salt tolerance

6 Salt tolerabilty Salt affect overall signal intensity water 50 mM Tris 10 mM MgCl 2 50 mM NaCl RNA Pico - old - 1 ng total RNA was run in different salt-containing media water 50 mM Tris 10 mM MgCl 2 50 mM NaCl Salt tolerance RNA 6000 Pico - Series II

7 Concentration accuracy - Rat heart Total RNA (in water) was run in 5 different concentrations (2x each per chip) on 12 replicate chips (6 instruments x 2 different chip lots) – All samples (and Ladder) were pipetted individually as with standard protocol. The quantitation calibration has been adjusted to better fit UV measures. Together with the more stable RNA ladder it provides for a more accurate and robust quantitation Quantitation

8 RIN reproducibility -Burkitt‘s Lymphoma Total RNA was run in 4 different conc. (1x each per chip) on 18 replicate chips (6 instruments x 3 different chip lots) -Mouse Ovary Total RNA (partially degraded) in 5 different conc. (2x each per chip) on 12 replicate chips (6 instruments x 2 different chip lots) Quantitation RNA Integrity Number (RIN) analysis is unchanged from old to new kits. The variability is within the expected range across the conc. range.

9 RNA 6000 Series II- Specifications Specifications RNA 6000 NanoRNA 6000 Nano Series II RNA 6000 PicoRNA 6000 Pico Series II Quantitative range Total RNA: 25–500 ng/µL mRNA: ng/µL Total RNA: 25–500 ng/µL mRNA: ng/µL Qualitative range Total RNA: 5–500 ng/µL mRNA: 25–250 ng/µL Total RNA: 5–500 ng/µL mRNA: 25–250 ng/µL Total RNA: 200–5000 pg/µL mRNA: pg/µL Total RNA: 50–5000 pg/µl (water) mRNA: 250–5000 pg/µl (water) Quantitation Reproducibility 10% CV 20% CV Quantitation accuracy 20% CV*30% CV* Buffer compatibility TE100 mM Tris or 125 mM NaCl or 15 mM MgCl2 TE50 mM Tris or 50 mM NaCl * ladder as sample

10 Former ProductsOrder NumberSeries II Products Order NumberUS Ref. Price ($) RNA 6000 Nano LabChip Kit RNA 6000 Nano Kit * RNA 6000 Nano Cooled Reagents RNA 6000 Nano Reagents * RNA 6000 Nano Ladder RNA 6000 Pico LabChip Kit RNA 6000 Pico Kit * RNA 6000 Pico Cooled Reagents RNA 6000 Pico Reagents * RNA 6000 Pico Ladder Ordering & pricing Former kits will be obsolete June 30, 2006 *New price reflects inclusion of the RNA ladder Ordering

11 Storage at 4C Storage at -20C Packaging & storage Packaging

12 SW update and assay download Prerequisites to use the Series II RNA kits: 2100 expert software B or B must be installed New RNA assays and firmware needs to be installed, use Assay Update Assay Update patch can be downloaded free of charge or requested on CD (fax return form included in every kit) Three options for software updates available: For biosizing users, free of charge update to the intro version of the 2100 bioanalyzer expert SW (Revision B.01.02) For B users, the upgrade to B is FREE of charge For all other users (i.e. beta versions), an update to the most current 2100 bioanalyzer expert SW (Revision B.02.02) for a minimal fee is recommended (order G2946CA)

13 Summary Improved RNA ladder now included in both RNA kits Consistently higher sensitivity for the RNA Pico assay Increased assay stability for samples with a high salt content for both RNA assays New quantitation calibration for both RNA assays Larger marker volumes included Shelf life initially increased to at least 4 months Reagents conveniently packaged according to storage temperatures New Assay Update CD available free of charge Only compatible with 2100 expert software B or B Offers for SW updates available More detailed information at