Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards.

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Presentation transcript:

Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards

Eggs (5 days to 1 year) Larva (~10 days) Pupa (1-2 days) Adult (1 week-2 months)

Vectors of human disease Infected blood meal Pathogens ingested Pathogens reproduce Next blood meal Pathogens transmitted through saliva Pathogens Ingested Pathogens Injected

Mosquitoes enjoying an artificial blood meal

Parafilm membrane Cow blood Feed mosquitoes

Esophagus : No carboxypeptidae gene expression Carboxypeptidase gene expression in the midgut cells Cow blood

Why did we think we had cloned a real; Carboxypeptidase A gene?

Fluorescent Anopheles gambiae mosquito larvae

Whole-mount in situ hybridization Control

Follicular Epithelium ~ 10hr PBM Vitelline envelope 15a-1: Eggshell Gene Vitelline envelope

Anti-LaCrosse Protein MxA Human Anti-Viral Gene Induced by IFN-  /  Inhibits virus transcriptase Other antiviral activities?

Micro-inject embryos with DNA constructs

SYBR green stained gel of mosquito genes expressed after a blood meal. PCR by Priscilla Mc. Dowell ‘01

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