The Relationship of Ultraviolet Light to the Growth of Micrococcus luteus and Serratia marcescens Catherine Francis 2009 Research Education for Teachers.

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Presentation transcript:

The Relationship of Ultraviolet Light to the Growth of Micrococcus luteus and Serratia marcescens Catherine Francis 2009 Research Education for Teachers Northeastern University, Boston, MA Claire Duggan / Program Director Serratia marcescens Micrococcus luteus The Relationship of Ultraviolet Light to the Growth of Micrococcus luteus and Serratia marcescens Catherine Francis 2009 Research Education for Teachers Northeastern University, Boston, MA

Essential questions 1. What are the characteristics of a prokaryotic organism? 2. Does ultraviolet light influence the growth and health of living organisms? 3. What sterile techniques are unique to microbiology?

Hypothesis If we expose pigmented bacteria to ultraviolet light, then the growth will be reduced.

Purpose High school biology students will determine if ultraviolet radiation influences the growth of pigmented bacteria. Upon successful completion of this lesson students will be able to grow bacteria on a petri dish using the quadrant streak method. The control group of bacteria will be grown without ultraviolet light while the experimental group will be grown with ultraviolet light from a germicidal UV lamp. After the bacteria is grown overnight in an incubator, the number of colonies will be counted in order to determine the influence of ultraviolet light.

Materials Safety Goggles Disposable Nitrile Gloves Micrococcus luteus (yellow) Serratia marcescens (red) Sterile prepoured plates of nutrient agar Sterile Toothpicks Germicidal Lamp Incubator Bleach

csiro.au/

Serratia marcescens

Serratia marcescens

Micrococcus luteus

References "Encyclopedia - Britannica Online Encyclopedia." Encyclopedia Britannica Online Encyclopedia. 21 July Gray, John. "PG.com." PG.com. 21 July "Home - The Skin Cancer Foundation." Home - The Skin Cancer Foundation. 21 July Lerner, Lee, and Brenda Wilmoth. "Ultraviolet Rays and Radiation." World of Earth Science 2 (2003): 605. Gale Virtual Reference Library. 21 July Wade, Jeffrey, and Richard Coffin. Molecular Studies of Ultraviolet Radiation Effects on Marine Bacteria. Pensacola: Center for Environmental Diagnostics and Bioremediation, University of West Florida, Zeman, Gary. "Health Physics Society." Health Physics Society. 21 July Zenoff, V, and M Farias. "Diverse Responses to UV-B Radiation and Repair Mechanisms of Bacteria Isolated from High Altitude Aquatic Environments." Applied and Environmental Microbiology 2.12 (2006):