Chapter 3 Diffusion and Osmosis

By the end of the lesson, you should be able to OBJECTIVES By the end of the lesson, you should be able to define and describe diffusion, osmosis and active transport to describe the idea of a partially permeable membrane assess the importance of water potential gradient in water uptake by plants explain the effects of osmosis in plant and animal tissues define and discuss importance of active transport in ion-uptake by root hairs and glucose uptake by cells in the villi

Introduction Recall: ~ A cell is the basic unit of life ~ Many complex reactions take place inside the cell ~This means that many raw materials must be brought into the cell ~ And new products formed by the chemical reactions, and waste materials, must exit the cell.

How? How do the raw materials get into the cell? How do the new products leave the cell? How are the waste materials removed from the cell? Answer: Materials move across the partially permeable cell membrane By processes called _________, _________ and _______________ diffusion osmosis active transport

Diffusion Diffusion is the net movement of ions or solute molecules from a region of higher concentration to a region of lower concentration, down a concentration gradient molecule moves from a region of high concentration to that of low concentration concentration gradient region of high concentration of molecule X region of low concentration of molecule X

Diffusion Diffusion in gases Gas molecules move from a region of higher concentration to that of a lower concentration B. The movement continues until they are evenly spread

Diffusion Diffusion in liquids A. B. Dissolved particles of a substance in a liquid move from a region where they are more concentrated to a region where they are less concentrated. The particles continue to move until they are evenly distributed.

Importance of Diffusion Initially, the concentration of a substance outside the cell is higher than inside the cell. The particles diffuse across the cell membrane. Later, an equal concentration of the substance inside and outside the cell is attained.

Importance of Diffusion Living cells continuously use up oxygen during aerobic respiration. The concentration of oxygen inside the cell falls. Oxygen molecules diffuse into the cell until the oxygen concentration is raised again. During respiration, carbon dioxide is produced. The concentration of carbon dioxide rises and creates a concentration gradient. carbon dioxide oxygen Oxygen is supplied to Amoeba by diffusion. Diffusion enables metabolic wastes such as carbon dioxide to be excreted out.

Diffusion Factors which Affect the Rate of Diffusion Temperature: An increase in temperature results in a higher rate of diffusion. Temp   Molecules move faster  Rate of diffusion  Size of particles: Small molecules or ions diffuse faster than large ones. Particle size  Energy to move particle   Rate of diffusion  Thickness of the barrier: Membranes of plant and animal cells are of about the same in thickness and permeability. Plant cell walls vary in their thickness. The thicker the cell walls, the slower the rate of diffusion. Thinner membrane  Higher rate of diffusion Concentration gradient: The greater the concentration gradient, the higher the rate of diffusion Steeper concentration gradient  Higher rate of diffusion Surface area: The rate of diffusion into a cell depends on the total surface area of the cell membrane.  surface area   rate of diffusion.

Lungs http://www.bio.psu.edu/Courses/Fall2002/Biol142/respiratory/respiratory.html

Diffusion in our body Recall: Our cells have a partially permeable membrane Partially permeable membrane Allows some substance through and not others How do these substances pass through? via DIFFUSION

Practical Application Kidney dialysis Waste products are removed from the blood by diffusing across artificial membrane in the machine Blood cells and other large protein are retained

selectively-permeable membrane Osmosis Osmosis is the diffusion of water across a selectively permeable membrane, from a high water potential to a low water potential. selectively-permeable membrane The selectively permeable membrane on the side of the water is being continuously bombarded by water molecules which pass through. This causes an increase in the volume of sucrose solution and a decrease in the volume of water. water molecules sucrose molecule water sucrose solution

Osmosis Water Potential The water potential of a solution is a measure of the tendency of water molecules to move from one place to another A dilute solution has a higher water potential than a concentrated solution The difference in water potential between two solutions is known as the water potential gradient

Osmosis Osmosis and Plant Cells In plant cells, the cell sap contains dissolved salts and sugar. If the cell sap has a lower water potential than that of the surrounding fluids, water from the outside enters by osmosis. Turgor pressure – The outward pressure which the cell sap exerts against the inside wall of the cell Turgor – A state when a cell is firm or turgid due to water entering the cell, causing it to swell.

Importance of Turgor in Plants Changes in turgor of the guard cell causes the opening of the stomata

Turgor (II) Changes in turgor of the pulvinus (small swelling at the base of the leaflets) causes the folding of leaflets in the Mimosa

Osmosis Osmosis and Plant Cells cell sap more concentrated than outside solution Turgid cells. The cytoplasm is pressed against the cell wall. The cell swells up and is prevented from bursting by the cellulose cell wall. water enters by osmosis cellulose cell wall plasma membrane cell sap less concentrated than outside solution water leaves by osmosis Plasmolysed cells. Water is lost from its vacuole, the cytoplasm shrinks and is withdrawn from the cell wall. cytoplasm

Plasmolysis leads to wilting

Fertilizer - Good or Bad? Too much fertilizer added will cause the plant to wilt Soil solution becomes very concentrated Water moves out of root Plant will wilt Sufficient water must be added to ensure plant survival

Osmosis Osmosis and Animal Cells normal red blood cell Changes observed under a microscope when blood is dropped into hypertonic and hypotonic solutions. in hypertonic solution in hypotonic solution cell shrinks cell swells cell becomes crenated cell bursts and cell is said to be haemolysed

carrier protein changes shape Active Transport Active transport is an energy-consuming process by which substances are transported from regions of low concentration to regions of high concentration against a concentration gradient. binding site substrate molecule OUTSIDE carrier protein cell membrane restoration INSIDE binding carrier protein changes shape substrate released energy from ATP used

Ion uptake by root hair is facilitated by active transport Plants need to absorb mineral salts from the soil. They are present as charged ions which cannot diffuse across partially-permeable membranes. soil particle high concentration of ions low concentration of ions Direction of active transport Ion uptake by root hair is facilitated by active transport

Uptake of glucose by cells in the villi Active Transport Active transport enables absorption of glucose and amino acids through the inner surface of the small intestine. The presence of microvilli increases the surface area over which active transport occurs. microvillus cell lining the villus Uptake of glucose by cells in the villi glucose molecule direction of active transport