Microscopy By Jane Horlings

Microscopy Robert Hooke, circa 1700sElectron Microscope, late 1900s

Early light microscope (UL), drawing by Hooke (LL) Light Microscopes

Dissecting (stereo) microscope (L), compound microscope (R)

Light Microscopy; Plant Cells Onion root tip, cell division (L), shoot tip (R)

Light Microscopy, Phase Contrast Single celled Amoeba dividing (L), green alga Micrasterias (R)

Phase contrast Fluorescence Confocal Light Microscopy

Electron Microscopes

Limitations of Electron Microscopy Works in a vacuum Specimens are dead, chemically preserved; no life processes can be seen No color (colorized by artist on computer)

Electron Microscopes Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM)

Scanning Electron Microscopy SEM of cilia TEM of cilia

Bacterium RBCs in clot SEM

Mite (UL) Gecko toes (LL) Shark skin (LR) SEM

Red blood cells and Trypanosoma (L), Giardia (R) SEM Trypanosome Red blood cells

Transmission Electron Microscopy SEM of cilia TEM of cilia

Viruses (L), animal cell (R) TEM

Animal cell (L), muscle tissue (R) TEM

Light Microscopy Based on light Specimens can be alive; life processes can be seen Color; dyes may be used

Parts of the Microscope Ocular lenses Objective lenses How to compute the magnification

Use of the Microscope Place slide in center Adjust light, lenses, barrel –Put on low magnification! –Move stage all the way up and then back down half a turn!

Use of the Microscope Look and readjust focus Move to higher magnification if needed

Use of the Microscope Importance of focusing with the fine adjustment! Oil immersion lens How to adjust the light

End