BIOCHEMICAL AND PATHOLOGICAL CHANGES OF THE LIVER OF ALBINO MICE (Mus musculus) WHEN EXPOSED TO SYNTHETIC TOXICANTS (Sodium hypoclorite, Zonroxand Albatross) Precious Mae Polloso Gereka Ozarraga

The Study The study basically tries to find out the survival rate of the albino mice (Mus musculus) when subjected to synthetic toxicants.

The Problems What are the changes in the liver of Mus musculus upon the administration of the different synthetic toxicants?

The Problems Sub-problems: What are the effects of each synthetic toxicants on the histology and gross morphology of the liver of Mus musculus? What are the responses or changes that took place in the liver to its exposure to the varying concentrations, 50% and 100%, of the synthetic toxicants? What are the differences in the behavior of the subject when it is exposed to Sodium hypochlorite, Zonrox bleach and Albatross?

The Scope This study was primarily designed to determine the biochemical and pathological changes of the liver of Mus musculus exposed to synthetic toxicants under laboratory observation. Sodium hypochlorite, Zonrox bleach and Albatross will be used as the synthetic toxicant. The mice (Mus musculus) will be exposed to similar concentrations of the said synthetic toxicants. A negative control set-up will be prepared without treatment.

Methodology Collection of Samples Preparation of Toxicants Exposure to Toxicants Observation Behavioral Analysis Preparetion of Organ for further testing Tests Observation and Analysis

The Results LEGEND: N – NormalH – HyperWO – Worn Out W – WeakerS – More SleepI - Inactive 100% Sodium hypochlorite 50% Sodium hypochlorite Albatross100% Zonrox bleach 50% Zonrox bleach Control Day 1 NNNNNN Day 2 NNNNNN Day 3 HHNHHN Day 4 W / WOHNWHN Day 5 W / SWOH N Day 6 W / SSWSSN Day 7 IIIIIN

The Results Weight of Liver

The Results Percentage of Lipid

The Conclusion Increasing the concentration caused the loss of biomolecules and decreased in percentage. It also causes prominent alteration of tissues and cell structures brought about by uptake of toxicants. Sodium hypochlorite have more pronounced affect than the other toxicants basing on the rate and degree of biological changes. Thus, it showed that the greater the amount of chemical taken up by an organism, the greater the toxic response.

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