UNDERSTANDING LINKAGE, AND GENETIC MAPPING. INTRODUCTION Each species of organism must contain hundreds to thousands of genes –Yet most species have at.

Slides:



Advertisements
Similar presentations
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. 4-1 Classical Genetics — Lecture I Dr. Steven J. Pittler.
Advertisements

1 LECTURE 5: LINKAGE. 2 Linked genes, recombination, and chromosomal mapping Mendel's Law of Independent Assortment is a consequence of the fact that.
UNDERSTANDING LINKAGE, AND GENETIC MAPPING. INTRODUCTION Each species of organism must contain hundreds to thousands of genes Yet most species have at.
Linkage genes and genetic recombination
Instructor: Dr. Jihad Abdallah Linkage and Genetic Mapping
PowerPoint Presentation Materials to accompany
Mutant phenotypes Short aristae Black body Cinnabar eyes Vestigial wings Brown eyes Long aristae (appendages on head) Gray body Red eyes Normal wings Red.
Biology Ch. 11 Review.
LINKAGE AND GENETIC MAPPING IN EUKARYOTES. In eukaryotic species, each linear chromosome contains a long piece of DNA A typical chromosome contains many.
KEY CONCEPT Genes can be mapped to specific locations on chromosomes.
April 2008 Mendelian Genetics Gene Linkage & Polyploidy April 9, 2008 Mr. Bromwell.
Linkage, Recombination and Eukaryotic Mapping. Outline Introduction Complete Linkage compared to independent assortment Crossing over with linked genes.
6- GENE LINKAGE AND GENETIC MAPPING Compiled by Siti Sarah Jumali Level 3 Room 14 Ext 2123.
Chapter 11 Introduction to Genetics. Genetics The study of the inheritance of traits.
10.4 Genes of Different Chromosomes Are Inherited Independently
Crossing Over of John Edward’s Chromosomes
Section 7.3: Gene Linkage & Mapping
Lesson Overview 11.4 Meiosis.
Data from trihybrid crosses can also yield information about map distance and gene order The following experiment outlines a common strategy for using.
Chapter 11 Review Section Assessments.
An early dihybrid cross
POST MENDELIAN GENETICS
Genetic recombination in Eukaryotes: crossing over, part 1 I.Genes found on the same chromosome = linked genes II.Linkage and crossing over III.Crossing.
You have body cells and gametes.
You have body cells and gametes.
Sexual Reproduction and Genetics
Genetica per Scienze Naturali a.a prof S. Presciuttini An early dihybrid cross In the early 1900s, William Bateson and R. C. Punnett were studying.
Lesson Overview Lesson OverviewMeiosis Chromosome Number Chromosomes—those strands of DNA and protein inside the cell nucleus—are the carriers of genes.
1900: Biology finally catches up with Mendel. Independently, Karl Correns, Erich von Tschermak, and Hugo de Vries all found that Mendel had explained the.
Linkage & Gene Mapping in Eukaryotes
The Chromosome Theory of Inheritance Chpt. 15 Chpt. 15.
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Chapter 10 Patterns of Inheritance.
 Linked Genes Learning Objective DOT Point: predict the difference in inheritance patterns if two genes are linked Sunday, June 05,
Chromosomal Patterns of Inheritance The Next Step – Relating Mendel to Genes.
1 LECTURE 5: LINKAGE. 2 Linked genes, recombination, and chromosomal mapping Mendel's Law of Independent Assortment is a consequence of the fact that.
Today: Chi squared and non- nuclear inheritance. Homologous pair of chromosomes Linkage can be used to determine distance.
The Experiments of Gregor Mendel Genetics – the study of heredity Mendel – studied ordinary pea plants The Role of Fertilization Pea plants – self-pollinating.
Introduction to Genetics Genetics- scientific study of heredity Gregor Mendel- father of genetics, laid the foundation of the science of genetics – Used.
PowerPoint Presentation Materials to accompany Genetics: Analysis and Principles Robert J. Brooker Copyright ©The McGraw-Hill Companies, Inc. Permission.
Genetics – Study of heredity is often divided into four major subdisciplines: 1. Transmission genetics, deals with the transmission of genes from generation.
Sexual Reproduction and Genetics Section 1: Meiosis Section 2: Mendelian Genetics Section 3: Gene Linkage and Polyploidy Chapter 10 Sexual Reproduction.
Pedigree Chart Symbols Male Female Person with trait.
1 Lecture 5. 2 Linked genes, recombination, and chromosomal mapping Mendel's Law of Independent Assortment is a consequence of the fact that chromosomes.
Aim: What is sex-linked inheritance?. Thomas Hunt Morgan was the first to associate a specific gene with a specific chromosome in the early 20th century.
1 THE WORK OF GREGOR MENDEL OBJECTIVES: 11.1 Describe how Mendel studied inheritance in peas. Summarize Mendel’s conclusion about inheritance. Explain.
Linkage -Genes on the same chromosome are called linked Human -23 pairs of chromosomes, ~35,000 different genes expressed. - average of 1,500 genes/chromosome.
Chromosomal Basis of Inheritance Linked Genes Sex-linked Genes.
GENERAL GENETICS Ayesha M. Khan Spring Linkage  Genes on the same chromosome are like passengers on a charter bus: they travel together and ultimately.
PROBABILITY AND STATISTICS The laws of inheritance can be used to predict the outcomes of genetic crosses For example –Animal and plant breeders are concerned.
Genetic map & crossing over. Linked assortment RrYy RY ry Possible gametes. IS NOT ALWAYS TRUE.
Recombination and Linked Genes
1 Copyright © 2016 McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education.
GENETIC MAPPING IN PLANTS AND ANIMALS
LINKAGE AND GENETIC MAPPING
GENETIC MAPPING IN PLANTS AND ANIMALS
Gene Mapping and Crossing Over –
Genetic Linkage.
Linked genes.
Gene Mapping in Eukaryotes
LINKAGE AND GENETIC MAPPING IN EUKARYOTES
The Chromosomal Basis of Inheritance
The Chi Square Test A statistical method used to determine goodness of fit Goodness of fit refers to how close the observed data are to those predicted.
And Yet more Inheritance
The Chi Square Test A statistical method used to determine goodness of fit Goodness of fit refers to how close the observed data are to those predicted.
Minot State University Genetics Biol 215
Mendelian Genetics chapter 10.1
The Chi Square Test A statistical method used to determine goodness of fit Goodness of fit refers to how close the observed data are to those predicted.
DIHYBRID CROSSES & GENE LINKAGE
Chapter 12 Linkage Maps. Chapter 12 Linkage Maps.
Presentation transcript:

UNDERSTANDING LINKAGE, AND GENETIC MAPPING

INTRODUCTION Each species of organism must contain hundreds to thousands of genes –Yet most species have at most a few dozen chromosomes Therefore, each chromosome is likely to carry many hundred or even thousands of different genes –The transmission of such genes will violate Mendel’s law of independent assortment 5-2 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5.1 LINKAGE AND CROSSING OVER In eukaryotic species, each linear chromosome contains a long piece of DNA –A typical chromosome contains many hundred or even a few thousand different genes The term linkage has two related meanings –1. Two or more genes can be located on the same chromosome –2. Genes that are close together tend to be transmitted as a unit 5-3 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

Chromosomes are called linkage groups –They contain a group of genes that are linked together The number of linkage groups is the number of types of chromosomes of the species –For example, in humans 22 autosomal linkage groups An X chromosome linkage group A Y chromosome linkage group Genes that are far apart on the same chromosome may independently assort from each other –This is due to crossing-over 5-4 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

Crossing Over May Produce Recombinant Phenotypes In diploid eukaryotic species, linkage can be altered during meiosis as a result of crossing over Crossing over –Occurs during prophase I of meiosis at the bivalent stage –Non-sister chromatids of homologous chromosomes exchange DNA segments Figure 5.1 illustrates the consequences of crossing over during meiosis 5-5 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5-6 Figure 5.1 The haploid cells contain the same combination of alleles as the original chromosomes The arrangement of linked alleles has not been altered

5-7 Figure 5.1 These haploid cells contain a combination of alleles NOT found in the original chromosomes These are termed parental or non- recombinant cells This new combination of alleles is a result of genetic recombination These are termed nonparental or recombinant cells

Bateson and Punnett Discovered Two Traits That Did Not Assort Independently In 1905, William Bateson and Reginald Punnett conducted a cross in sweet pea involving two different traits –Flower color and pollen shape This is a dihybrid cross that is expected to yield a 9:3:3:1 phenotypic ratio in the F 2 generation –However, Bateson and Punnett obtained surprising results Refer to Figure Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

Figure A much greater proportion of the two types found in the parental generation

Bateson and Punnett Discovered Two Traits That Did Not Assort Independently They suggested that the transmission of the two traits from the parents was somehow coupled –The two traits are not easily assorted in an independent manner However, they did not realize that the coupling is due to the linkage of the two genes on the same chromosome 5-10 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

Morgan Provided Evidence for the Linkage of Several X-linked Genes The first direct evidence of linkage came from studies of Thomas Hunt Morgan Morgan investigated several traits that followed an X-linked pattern of inheritance Figure 5.3 illustrates an experiment involving three traits –Body color –Eye color –Wing length 5-11 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5-12 Figure 5.3

5-13 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display Morgan observed a much higher proportion of the combinations of traits found in the parental generation P Males P Females Morgan’s explanation: All three genes are located on the X chromosome Therefore, they tend to be transmitted together as a unit

Morgan Provided Evidence for the Linkage of Several X-linked Genes However, Morgan still had to interpret two key observations –1. Why did the F 2 generation have a significant number of nonparental combinations? –2. Why was there a quantitative difference between the various nonparental combinations? 5-14 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5-15 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display Gray body, red eyes1,159 Yellow body, white eyes1,017 Gray body, white eyes 17 Yellow body, red eyes 12 Total2,205 Let’s reorganize Morgan’s data by considering the pairs of genes separately Red eyes, normal wings 770 White eyes, miniature wings 716 Red eyes, miniature wings 401 White eyes, normal wings 318 Total2,205 It was fairly common to get this nonparental combination But this nonparental combination was rare

To explain these data, Morgan considered the previous studies of the cytologist F.A Janssens Janssens had observed chiasmata microscopically –And proposed that crossing over involves a physical exchange between homologous chromosomes Morgan shrewdly realized that crossing over between homologous X chromosomes was consistent with his data Crossing over did not occur between the X and Y chromosome –The three genes were not found on the Y chromosome 5-16 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

Morgan made three important hypotheses to explain his results –1. The genes for body color, eye color and wing length are all located on the X-chromosome They tend to be inherited together –2. Due to crossing over, the homologous X chromosomes (in the female) can exchange pieces of chromosomes This created new combination of alleles –3. The likelihood of crossing over depends on the distance between the two genes Crossing over is more likely to occur between two genes that are far apart from each other Figure 5.4 illustrates how crossing over provides an explanation for Morgan’s trihybrid cross 5-17 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5-18 Figure 5.4 These parental phenotypes are the most common offspring because the genes are far apart These recombinant offspring are not uncommon

5-19 Figure 5.4 because the genes are very close together These recombinant offspring are fairly uncommon These recombinant offspring are very unlikely 1 out of 2,205

Chi Square Analysis This method is frequently used to determine if the outcome of a dihybrid cross is consistent with linkage or independent assortment Let’s consider the data concerning body color and eye color An example of a chi square approach to determine linkage is shown next 5-20 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5-21 Step 1: Propose a hypothesis. The genes for eye color and body color are X-linked and, somehow, independently assorting Even though the observed data appear inconsistent with this hypothesis, the hypothesis allow us to calculate expected values Indeed, we actually anticipate that the chi square analysis will allow us to reject this hypothesis in favor of a linkage hypothesis Step 2: Based on the hypothesis, calculate the expected values of each of the four phenotypes. An independent assortment hypothesis predicts that each phenotype has an equal probability of occurring Refer to Punnett square on the next slide

5-22 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display Total offspring equals 2,205 Therefore, the expected number of each phenotype is 1/4 X 2,205 = 551

Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display 5-23 Step 3: Apply the chi square formula    (O 1 – E 1 ) 2 E1E1 (O 2 – E 2 ) 2 E2E2 (O 3 – E 3 ) 2 E3E3 (O 4 – E 4 ) 2 E4E4 +++    (1159 – 551) (17 – 551) (12 – 551) (1017 – 551)       2,109.8

Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display 5-24 Step 4: Interpret the calculated chi square value This is done with a chi square table as shown in Chapter 2 There are four experimental categories (n = 4) Therefore, the degrees of freedom (df) is n -1 = 3 The calculated chi square value is enormous Thus, the deviation between observed and expected values is very large According to Table 2.1, such a large deviation is expected to occur by chance alone less than 1% of time Therefore, we reject the hypothesis that the two genes assort independently In other words, we conclude that the genes are linked

5.2 GENETIC MAPPING IN PLANTS AND ANIMALS Genetic mapping is also known as gene mapping or chromosome mapping Its purpose is to determine the linear order of linked genes along the same chromosome Figure 5.8 illustrates a simplified genetic linkage map of Drosophila melanogaster 5-42 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display

5-43 Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display Figure 5.8 Each gene has its own unique locus at a particular site within a chromosome

5-45 Experimentally, the percentage of recombinant offspring is correlated with the distance between the two genes –If the genes are far apart  many recombinant offspring –If the genes are close  very few recombinant offspring Map distance = Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display Number of recombinant offspring Total number of offspring X 100 The units of distance are called map units (mu) They are also referred to as centiMorgans (cM) One map unit is equivalent to 1% recombination frequency

5-47 Figure 5.9 Chromosomes are the product of a crossover during meiosis in the heterozygous parent Recombinant offspring are fewer in number than nonrecombinant offspring

5-48 The data at the bottom of Figure 5.9 can be used to estimate the distance between the two genes Map distance = Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display Number of recombinant offspring Total number of offspring X X 100= = 12.3 map units