11 5/19/2015 Parvo Viruses

22 5/19/2015 Parvo Viruses l Structure l Classification l Multiplication l Clinical manifestations l Epidemiology l Diagnosis l Control Baron’s Web Site Baron’s Web Site

33 5/19/2015 Parvovirus Structure l Small icosahedral l non enveloped l ssDNA + or - strand Unable to grow Parvovirus in vitro cell culture

44 5/19/2015 Parvovirinae l Genus Parvovirus –Type Species mice minute virus l Genus Erythrovirus –Type Species B19 virus Web siteWeb site l Genus Dependovirus –Type Species adeno-associated virus 2

55 5/19/2015 Classification l Family Parvoviridae –Subfamily Parvovirinae –Subfamily Densovirinae

66 5/19/2015 Densovirinae l Genus Densovirus –Type Species Junonia coenia densovirus l Genus Iteravirus –Type Species Bombyx mori densovirus l Genus Contravirus –Type Species Aedes aegypti densovirus

77 5/19/2015 Paroviridae Classification old l Parvovirus –Parvo Virus Like Agent –Serum Parvo Like Virus l Dependovirus –AKA Adeno Associated Virus l Insect virus

88 5/19/2015 B19 Genome Parvovirus B19 Genome VP1 Coding Region VP2 Region

99 5/19/2015 Clinical manifestations l Mild flu like illness l Erythema infectosum l Aplastic Crisis l Stillbirth Web SiteWeb Site

10 5/19/2015 Mild flu like illness l Fever l Headache l Chills l Myalgia, l Malaise l Sore throat l hemoglobin conc down

11 5/19/2015 Erythema infectosum l Fifth Disease l Rash on face that spreads to exposed skin eg arms and legs l Subsides over 2 weeks l Relapses frequent l Arthritis of hands, wrists, knees and ankles

12 5/19/2015 Typical facial exanthem of erythema infectiosum. Note the sparing of the perioral areas, resulting in the typical "slapped cheek” appearance. Web Site

13 5/19/2015 Lacy, reticular exanthem of the extremity in a child with erythema infectiosum. Web site

14 5/19/2015 Aplastic Crisis l patients with Sickle cell anemia ie chronic hemolytic anemia l Fever, malaise, myalgia, chills l Maculopapular rash –arthritis & joint swelling, itching l Decreased RBC formation – Life threatening Web site

15 5/19/2015 Stillbirth l Parvovirus can cross the placenta l no congenital abnormalities

16 5/19/2015 Fifth Disease l Droplets inhaled into upper respiratory tract l local replication l Viremia l Replication in upper respiratory tract l Rash and arthalgia

17 5/19/2015 Aplastic Crisis l Droplets inhaled into upper respiratory tract l virus replicates in bone marrow l Reticulocytosis

18 5/19/2015 Clinical manifestation of B19 Web site

19 5/19/2015 Web site

20 5/19/2015 Epidemiology l Most infections before 40 years l Erythema infectiosum 4-15 years l 65% of adults have antibodies

21 5/19/2015 l Spread by respiratory droplets l less contagious than measles

22 5/19/2015 Diagnosis l Virus detected in serum or throat wash –Electron microscopy –EIA, RIA –DNA hybridization l Anti viral IgM in serum – easier

23 5/19/2015 Control l No specific control l patients with clinical erythema infectiosm not infectious –already transmitted the virus

24 5/19/2015 DONE!!!