Launching MIURA sample prep system The next generation of automation DSP SYSTEMS Information : mail@dspsystems.eu or +31-344-846303 Internet: www.dspsystems.eu

Introduction The Full-Automatic Sample Preparation System for dioxins and PCBs analysis has three features… High Performance Clean-up User-friendly Column Design Unique way of flow switching Main advantages Low solvent consumption Hexane 90 ml Toluene 4 ml Run-time 90 minutes No cross contamination Columns easy to connect and no leakage Competitive prices

Availability and Service The system is in Europe and several other countries available via DSP Systems as : GO-2HT capable of running two samples parallel GO-4HT capable of running four samples parallel GO-6HT capable of running six samples parallel Installation, service and maintenance will be supplied by DSP Systems A full maintenance contract is optional with a response time of 24 hours for customers within a radius of 300 km from the middle of the Netherlands and 48 hours within the rest of the EU countries

General Dioxin method Sample Sample extraction or dilution Clean-up 16/04/2017 General Dioxin method Sample ← spike with 13C labelled standards Sample extraction or dilution concentration Clean-up ← add Control standard ← add Recovery standards concentration GC-HRMS or GC-MS/MS Required time 1-2 days

Background --- Features of New System High performance clean-up User-friendly column design Unique way of flow switching without valves

Background --- Features of New System High performance clean-up 4 columns Silver nitrate Si column Sulfuric acid Si column Carbon column Alumina column Heated Purification and elution Enhances dioxins elution speed Accelerate a chemical reaction User-friendly column design … Unique way of flow switching without valves

Background --- Features of New System High performance clean-up User-friendly column design Easy applying sample Easy connection Disposable parts No cross contamination No washing is required Clap! Clap! Clap!

Background --- Features of New System High performance clean-up 90 mL of hexane for eluting dioxins and PCBs: Trapping dioxins and NO-PCBs on carbon Trapping MO and NDL-PCBs on alumina Open the pinch valve Elution PCBs with 1.5 ml of toluene Close the pinch valve and open the other pinch valve Elution Dioxins with 1 ml of toluene

First results Recovery in real samples Sample type: PFAD Fish oil Mix of fatty acid Minerals Compound feed

First results PeCDD trace peak of interest is 1,2,3,7,8 *1 sample tocopherol Result after classical clean-up Result after clean-up using the new system *1 TEF = 1,0

First results HxCDF trace peak of interest is 1,2,3,5,7,8; sample tocopherol Result after classical clean-up Result after clean-up using the new system

60mL 100mL 1.5hrs 1.5hrs 1 hr GC-MSMS GC-HRMS Conclusion Extraction Purification Measurement GC-MSMS 1.5hrs 1.5hrs 1 hr GC-HRMS 60mL 100mL Multiple sample extraction Full Automatic Sample preparation System GC-MSMS or/and GC-HRMS High sample Throughput Eco-friendly analysis Results available within 8 hours

DSP SYSTEMS Information : info@dspsystems.eu or +31 6-1366 4946 More information Chris.van.Wakeren@dspsystems.eu Wim.Traag@dspsystems.eu Mail@dspsystem.eu Phone : +31-344-846303 DSP SYSTEMS Information : info@dspsystems.eu or +31 6-1366 4946