Nucleic acids 2006-2007 Nucleic Acids Information storage.

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Presentation transcript:

Nucleic acids

Nucleic Acids Information storage

proteins DNA Nucleic Acids Function:  genetic material stores information  genes  blueprint for building proteins DNA  RNA  proteins transfers information  blueprint for new cells  blueprint for next generation

A A A A T C G C G T G C T

Nucleic Acids Examples:  RNA (ribonucleic acid) single helix  DNA (deoxyribonucleic acid) double helix Structure:  monomers = nucleotides RNADNA

♦ DNA (deoxyribonucleic acid) – Ribose containing nucleic acid without oxygen ♦ RNA (ribonucleic acid) – Ribose containing nucleic acid with oxygen

Nucleotides 3 parts  nitrogen base (C-N ring)  pentose sugar (5C) ribose in RNA deoxyribose in DNA  phosphate (PO 4 ) group Nitrogen base I’m the A,T,C,G or U part!

Types of nucleotides 2 types of nucleotides  different nitrogen bases  purines double ring N base adenine (A) guanine (G)  pyrimidines single ring N base cytosine (C) thymine (T) uracil (U)

Nucleic polymer Backbone  sugar to PO 4 bond new base added to sugar of previous base polymer grows in one direction  N bases hang off the sugar-phosphate backbone Dangling bases? Why is this important?

Pairing of nucleotides Nucleotides bond between DNA strands  H bonds  purine :: pyrimidine  A T 2 H bonds  G C 3 H bonds Matching bases? Why is this important?

DNA molecule Double helix  H bonds between bases join the 2 strands A T C G H bonds? Why is this important?

Copying DNA Replication  2 strands of DNA helix are complementary have one, can build other have one, can rebuild the whole Matching halves? Why is this a good system?

When does a cell copy DNA? When in the life of a cell does DNA have to be copied?  cell reproduction mitosis  gamete production meiosis

DNA replication “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” James Watson Francis Crick 1953

Watson and Crick … and others… 1953 | 1962

Maurice Wilkins… and… 1953 | 1962

Rosalind Franklin ( )

Interesting note… Ratio of A-T::G-C affects stability of DNA molecule  2 H bonds vs. 3 H bonds  biotech procedures more G-C = need higher T° to separate strands  high T° organisms many G-C  parasites many A-T (don’t know why)

Another interesting note… ATP Adenosine triphosphate ++  modified nucleotide  adenine (AMP) + P i + P i